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Showing: 41 - 60 of 64 results

  4. pETDUET-GST AO7RE ∆238-245

    Plasmid
    #139318
    Purpose
    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria
    Depositor
    Allan Weissman
    Article
    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3
    Insert
    AO7 aa 126-258 (RNF25 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    AO7 cloned into the first multiple cloning site o…
    Promoter
    T7 promoter-1
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    139318-plasmid-map-sequence-id-275576

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  5. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid
    #207357
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207357-plasmid-map-sequence-id-408418

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  6. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid
    #207356
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207356-plasmid-map-sequence-id-408417

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  7. pRRLSIN.cPPT.RFPL4b.Luciferase.WPRE

    Plasmid
    #69252
    Purpose
    Luciferase in lentivirus backbone to report Dux4 activation of RFPL4b promoter
    Depositor
    Stephen Tapscott
    Insert
    RFPL4b (RFPL4B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    RFPL4b
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    69252-plasmid-map-sequence-id-123983

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  8. pGL3 RFPL4b promoter

    Plasmid
    #69250
    Purpose
    Luciferase reporter for Dux4 activation of RFPL4b transcription
    Depositor
    Stephen Tapscott
    Insert
    RFPL4b (RFPL4B Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    RFPL4b
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. 6CVZ (RFWD3)

    Plasmid
    #119964
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    RFWD3 (RFWD3 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119964-plasmid-map-sequence-id-232688

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  20. pFBD-BirA_RFWD3:425-774

    Plasmid
    #210884
    Purpose
    Insect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    RFWD3:S425-E774 (RFWD3 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210884-plasmid-map-sequence-id-424500

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Showing: 41 - 60 of 64 results