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Plasmid#183136PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056K
Plasmid#183137PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opie2-PuroR[dsxM]
Plasmid#131616PurposeExpresses a functional PuroR only in males and dsRed in both genders.DepositorInsertsUseTagsExpressionInsectMutationPromoterOpie2 and hr5Ie1Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA[Tra].1026G
Plasmid#112689Purposeexpress gRNA targeting Tra under dU6-3 promoterDepositorInsertU6.3-gRNA[Tra] (tra Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pmScarlet-N1
Plasmid#188032PurposeExpresses CapChR2-mScarlet in mammalian cellsDepositorInsertCapChR2-mScarlet
UseTagsmScarletExpressionMammalianMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSGV1-1D3-28Z.1-3 mut
Plasmid#107227PurposeAnti-mouse CD19 CAR with CD3 ITAMs mutatedDepositorInsert1D3-28Z. 1-3 mut
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-dsRed-shscramble
Plasmid#71384PurposeExpresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cellsDepositorInsertCMV promoter-dsRed-mir30-shscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterCMV enhancer/promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-1b-RFC140ΔN555
Plasmid#175043PurposeOverexpression of human (Rfc1) p140ΔN555 in E.coliDepositorInsertReplication factor C subunit 1 (RFC1 Human)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only