Addgene

We narrowed to 58,002 results for: CaS;

Showing: 581 - 600 of 58002 results

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pnCasPA-BEC

Plasmid

#113349

Purpose

A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW SpCas9

Plasmid

#131506

Purpose

lentiviral expression of SpCas9 under hUBC promotor

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

SpCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP

Plasmid

#172221

Purpose

Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g3

Plasmid

#80786

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pZ8-T_dCas9

Plasmid

#74062

Purpose

pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23109

Plasmid

#113151

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

dCas9

Use

Tags

Expression

Bacterial

Mutation

D10A & H840A

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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DNMT3A-dCas9

Plasmid

#100090

Purpose

DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

DNMT3A (DNMT3A Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3A-dCas9-NLS

Expression

Mammalian

Mutation

includes aa 602-912

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8

Plasmid

#11817

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 (CASP8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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RCAS GFP (CT#22)

Plasmid

#13878

Depositor

Connie Cepko

Article

Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Insert

GFP

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-1

Plasmid

#129726

Purpose

Expressing SpCas9 and sgAAVS1-1

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-1

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV immediate-early enhancer/promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pSCAR_Cas9-hygro_GFP

Plasmid

#162075

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-hygromycin B phosphotransferase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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G13-CASE

Plasmid

#168127

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at F125/D126 within GNA13

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

Tags

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-zCas9

Plasmid

#101739

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9-UGI-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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