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pRK5_VHH05-EGFP-NPM1

Plasmid

#238268

Purpose

For overexpression of VHH05-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-NUP98-KDM5A-KS-FtoA

Plasmid

#238295

Purpose

For overexpression of EGFP-NUP98-KDM5A-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-NUP98-KDM5A-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-NUP98-KDM5A-KS

Plasmid

#238294

Purpose

For overexpression of EGFP-NUP98-KDM5A-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-NUP98-KDM5A-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-linker-KS-FtoA

Plasmid

#237687

Purpose

For overexpression of 52K-GFP-linker-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-linker-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-biotin-myc- mCE

Plasmid

#82475

Purpose

Expresses mRNA capping enzyme containing N terminal biotin and myc tag

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

Biotin (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

Plasmid

#80325

Purpose

Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Article

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Tags

Biotin

Expression

Mammalian

Mutation

H170A and H256A

Promoter

CMV

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT

Plasmid

#238281

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Plasmid

#238282

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-NPM1-gRNA for U2OS

Plasmid

#238250

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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TRE3G-mCherry-P2A-T2A-nanobody-KS

Plasmid

#238287

Purpose

For integration of mCherry-P2A-T2A-nanobody-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2A-T2A-nanobody-KS

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-mCherry-P2A-T2A-nanobody-KS-FtoA

Plasmid

#238288

Purpose

For integration of mCherry-P2A-T2A-nanobody-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2A-T2A-nanobody-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-IgKL-mNG

Plasmid

#183467

Purpose

To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-mNG

Plasmid

#183465

Purpose

To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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VZV ORF 60 (gL)

Plasmid

#64082

Purpose

eukaryotic expression plasmid for VZV glycoprotein L

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein L

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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VZV ORF 37(gH)

Plasmid

#64081

Purpose

eukaryotic expression plasmid for VZV glycoprotein H

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein H

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-Blast SHMT2res

Plasmid

#106301

Purpose

Expresses SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Silent mutations destroy sgRNA targeting sites

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUS2

Plasmid

#135102

Purpose

Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL50

Plasmid

#135101

Purpose

Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL23

Plasmid

#135104

Purpose

Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Tags

HA-Strep-Strep (N terminal on backbone)

Expression

Mammalian

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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