We narrowed to 1,102 results for: cas12a

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EC70188

Plasmid

#209449

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140230

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Publication

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg (reverse orientation)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P11

Plasmid

#120022

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P12

Plasmid

#120023

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-13

Plasmid

#119992

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Publication

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EC70196

Plasmid

#209457

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-23

Plasmid

#120002

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

Plasmid

#115218

Purpose

Expresses PguCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PguCas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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EC70364

Plasmid

#209515

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Publication

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW302-AcrVA4

Plasmid

#115656

Purpose

Expresses AcrVA4 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRDA_886

Plasmid

#201162

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

Depositor

John Doench

Publication

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBE50

Plasmid

#174657

Purpose

CAPTURE method receiver plasmid for Bacillus cloning and heterologous expression

Depositor

Huimin Zhao

Publication

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P31

Plasmid

#120042

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

Plasmid

#115213

Purpose

Expresses RanCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

RanCas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGBScomp High

Plasmid

#223202

Purpose

Group B Streptococcus High Expression Modular Complementation Vector

Depositor

Thomas Hooven

Publication

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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gCH29 (crCD81-1)

Plasmid

#217344

Purpose

crRNA targeting CD81

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD81-1 (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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