-
Plasmid#188802PurposeChimeric Avs4 with transmembrane effectorDepositorInsertAvs4 chimera
UseTagsExpressionBacterialMutationPromoterEcAvs4 native promoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Avs4_Archaeon_isolate_HyVt-335
Plasmid#188800PurposeAvs4 from Archaeon isolate HyVt-335DepositorInsertAvs4
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF1 (WT)
Plasmid#175414PurposeEncodes His-MBP-TEV-ybbR-WT eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-TetO-GFP_ColE1
Plasmid#171660PurposeQUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.DepositorInsertQUAS-5-T7-TetO-GFP ssra-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG017 (AVAST type 1)
Plasmid#157895PurposeExpresses the AVAST type 1 defense systemDepositorInsertAVAST type 1 (STAND ATPase with N-terminal serine protease)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF3∆165N (WT)
Plasmid#175418PurposeEncodes His-MBP-TEV-WT eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
UseTagsHis-MBP-TEVExpressionBacterialMutation∆165NPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLMB757
Plasmid#83792PurposeFructose biosensor plasmidDepositorInsertRegion upstream RL0489 (frcB) containing all of RL0488 (frcR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB713
Plasmid#83785PurposeSalicylic acid biosensor plasmidDepositorInsertRegion upstream RL1329 (salA) containing all of RL1328 (salR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB577
Plasmid#83778PurposePhenylalanine biosensor plasmidDepositorInsertpromoter of RL1860
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF3∆165N (WT)
Plasmid#175419PurposeEncodes His-MBP-TEV-ybbR-WT eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutation∆165NPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLMB686
Plasmid#83780Purposemyo-Inositol biosensor plasmidDepositorInsertupstream region of RL4655 (intA)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB684
Plasmid#83779PurposeXylose biosensor plasmidDepositorInsertupstream region of RL2720 (rbsC)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB687
Plasmid#83781PurposeSucrose biosensor plasmidDepositorInsertRegion upstream pRL120556 containing all of pRL120555
UseTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB708
Plasmid#83782PurposeTartrate biosensor plasmidDepositorInsertRegion upstream RL0996 containing all of RL0997
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB710
Plasmid#83783PurposeFormate biosensor plasmidDepositorInsertRegion upstream RL4393 (fdsG) containing all of RL4394
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only