We narrowed to 1,166 results for: ncl

1. 1455_pDEST_miniTol2_R4-R2_MCS

   Plasmid

   #171794

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGImC_Empty

   Plasmid

   #92015

   Purpose

   Empty backbone, includes IRES-mCherry

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSETT4gap

   Plasmid

   #153413

   Purpose

   φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

   Depositor

   Joern Kalinowski

   Article

   Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gapDH promoter from Eggerthella lenta

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-TEV-pro-conA

   Plasmid

   #159527

   Purpose

   Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

   Depositor

   Joshua Mylne

   Article

   Nonis et al bioRxiv. 2020.

   Insert

   Canavalia ensiformis pro-concanavalin A
   

   Use

   Tags

   Six-Histidine tag and tobacco etch virus (TEV) pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET3C-ARL16(CO)-His6

   Plasmid

   #216485

   Purpose

   Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

   Depositor

   Richard Kahn

   Insert

   ADP-ribosylation factor-like 16 (ARL16 Human)
   

   Use

   Tags

   includes GSGSGSHHHHHH (His6) tag at C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSXND1-hPPT1

   Plasmid

   #85817

   Purpose

   Expression of hPPT in mammalian cells

   Depositor

   Sandra Hofmann

   Article

   Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

   Insert

   human PPT1 (PPT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pME-EGFP-P2A-APEX2-NS

   Plasmid

   #108895

   Purpose

   Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   EGFP-P2A-APEX2
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLBH546_Tet-Cas14b1Locus

   Plasmid

   #112503

   Purpose

   Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLBH547_Tet-Cas14b2Locus

   Plasmid

   #112504

   Purpose

   Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS_KS_attB2_SA(0)_T2AGAL4

    Plasmid

    #125211

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ac5-STABLE2-blast

    Plasmid

    #208034

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS_KS_attB2_SA(2)_T2AGAL4

    Plasmid

    #125213

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS_KS_attB2_SA(1)_T2AGAL4

    Plasmid

    #125212

    Purpose

    Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBluescriptKS(-)-FRB

    Plasmid

    #104475

    Purpose

    Plasmid carrying FRB with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Expression

    Mutation

    includes only the FRB domain

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Halo-Erk2 donor

    Plasmid

    #113104

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    N terminal Erk2 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDR1-221

    Plasmid

    #69481

    Purpose

    Deletion Construct

    Depositor

    Kathleen Scotto

    Article

    Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

    Insert

    MDR1 promoter (ABCB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Insert includes MDR1 promoter sequence from -221 …

    Promoter

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Ac5-STABLE1-puro

    Plasmid

    #208031

    Purpose

    Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KG#45

    Plasmid

    #110926

    Purpose

    Vector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegans

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    hsp-16-2 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. YIplac211-RPL13A-FKBPx2

    Plasmid

    #104473

    Purpose

    Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    RPL13Ap (RPL13A Budding Yeast)
    

    Use

    Tags

    FKBPx2-HA

    Expression

    Yeast

    Mutation

    includes the last 354 bp of RPL13A only

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBluescriptKS(-)-FRBx2

    Plasmid

    #104476

    Purpose

    Plasmid carrying FRBx2 with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRBx2 (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Expression

    Mutation

    includes only the FRB domain

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge