We narrowed to 1,853 results for: rigi

1. mApple-proα2(I)-G610C

   Plasmid

   #119841

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

   Plasmid

   #240228

   Purpose

   Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

   Plasmid

   #240229

   Purpose

   Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAS-hhN-HA

   Plasmid

   #240236

   Purpose

   Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   hedgehog N-terminal fragment (hh Fly)
   

   Use

   Tags

   HA-spGFP11

   Expression

   Insect

   Mutation

   aa 1-257

   Promoter

   UAS

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. macroH2A2-GFP (pc2191)

   Plasmid

   #223709

   Purpose

   Expression of macroH2A2 tagged N-terminal to GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. macroH2A1.2-F192V-GFP (pc5115)

   Plasmid

   #223438

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.2-F192V (Macroh2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of phenylalanine 192 to valine (F192V)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-ND-Cdt1Δ499-546-HA-Puro

   Plasmid

   #193768

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1Δ499-546 (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. macroH2A2-V192F-GFP (pc5116)

   Plasmid

   #223439

   Purpose

   Mutation of valine 192 to phenylalanine (V192F) in macroH2A2.

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-F192V (MACROH2A2, H2AFY2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of valine 192 to phenylalanine (V192F)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Escherichia coli DH10B-MOB

    Bacterial Strain

    #229395

    Purpose

    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Bacterial Resistance

    60 ug/ml of dap

    Species

    Escherichia coli

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

12. XanthoMoClo Xanthobacter Genetic Toolkit

    Plasmid Kit

    #1000000259

    Purpose

    Use this modular cloning kit to assemble plasmids for expression in Xanthobacter and Roseixanthobacter.

    Depositor

    Michael Springer

    Available Since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

13. pStABK1

    Plasmid

    #193729

    Purpose

    Start-Stop assembly level 1 vector, BBR1 origin, kanR

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. VfAU1-LOV_136

    Plasmid

    #63632

    Purpose

    VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site

    Depositor

    Harald Janovjak

    Insert

    VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pfNL43-dE-EGFP

    Plasmid

    #36865

    Depositor

    Mario Chin

    Insert

    fNL43-dE-EGFP
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    inserted EGFP after 6350 nucleotide of original N…

    Promoter

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLSU1/o35S[]:MCS:NosT

    Plasmid

    #212163

    Purpose

    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (original 35sCAMV)

    Depositor

    Wayne Curtis

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAC01A

    Plasmid

    #193730

    Purpose

    Expresses deactivated-Cas9 (J23101) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRAC07A

    Plasmid

    #193733

    Purpose

    Expresses deactivated-Cas9 (J23107) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MYR-VfAU1-LOV_135

    Plasmid

    #63631

    Purpose

    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a MYR signal and a restriction site

    Depositor

    Harald Janovjak

    Insert

    MYR-VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-EcoR1-IRES-GFP

    Plasmid

    #66956

    Purpose

    original retroviral vector with one single EcoR1 cloning site

    Depositor

    Martine Roussel

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only