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pSYC-102

Plasmid

#74790

Purpose

pCS4+-NLS-EGFP-T2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

NLS-EGFP-T2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBS ZfHoxb1a

Plasmid

#27519

Depositor

Victoria Prince

Insert

Hoxb1a (hoxb1a Zebrafish)

Use

Riboprobe synthesis

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pDRAV-4

Plasmid

#26749

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pKS2

Plasmid

#24625

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP-miR-E1-1int

Plasmid

#19823

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

miRNA

Use

RNAi

Expression

Mammalian

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pBAD24MevT

Plasmid

#17820

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

MevT

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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PM-RA

Plasmid

#209878

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry-mEGFP

Plasmid

#127815

Purpose

Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mNeongreen-T2A-mCherry

Plasmid

#125698

Purpose

Produces equimolar expression of mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mNeongreen-T2A-mCherry

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Th

Plasmid

#105988

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

tyrosine hydroxylase (Th Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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mKO2-2A-mTurquoise2

Plasmid

#98838

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKO2-2A-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pRSETb

Plasmid

#67770

Purpose

Kohinoor for bacterial expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Dendra2-FTR-952

Plasmid

#57719

Purpose

Localization: Cytoskeleton, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

FTR

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHyVec12

Plasmid

#51851

Purpose

Expression in Hydra for RNAi

Depositor

Haifan Lin

Article

Juliano et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Inserts

GFP hairpin
DsRed2

Use

RNAi; Hydra expression

Promoter

Actin

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAM1706

Plasmid

#40242

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

PpsbAI

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57 iLOV CHLAMY

Plasmid

#26769

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Cloning vector

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only
pGP-AAV-GFAP-iGABASnFR2-WPRE

Plasmid

#218871

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

GFAP

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-5xTetO-pEF-H2B-Citrine-SV40

Plasmid

#186966

Purpose

Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

5xTetO-pEF-H2B-Citrine-SV40

Use

Lentiviral

Expression

Mammalian

Promoter

5xTetO-pEF-1alpha

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 (AAV5)

Viral Prep

#98932-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 (#98932). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent glutamate sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only