We narrowed to 20,259 results for: INO

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PKM-IRES-hrGFP

Plasmid

#73405

Purpose

Mammalian expression of mouse PKM and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKM (Prkcz Mouse)

Tags

3xFLAG and pIRES-hrGFP-2a

Expression

Mammalian

Mutation

truncated, constitutively active form

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E317K)

Plasmid

#29528

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 317 to lysine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pTK165_Mem-mCherry-4.1G-CTD

Plasmid

#46362

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Tags

Mem (N-terminal 20 amino acids of neuromodulin fo…

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

Plasmid

#32736

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
p7612 MSCV-P C-FlagHA 16E7 E10K

Plasmid

#163303

Purpose

Expresses HPV16 E7 E10K

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 E10K

Use

Retroviral

Tags

FlagHA

Mutation

E10K

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

Plasmid

#47453

Purpose

epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (N136,Grm2,C63)-cib1

Use

AAV; Tale

Tags

HA

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos4b-V69T

Plasmid

#98572

Purpose

photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos4b-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 ERbeta(144-530)

Plasmid

#35566

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

GST

Expression

Bacterial

Mutation

residues 144-530

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

Plasmid

#13616

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65, Thr 69 and Ser 70 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 ERbeta(144-255)

Plasmid

#35565

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

GST

Expression

Bacterial

Mutation

residues 144-255

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223 GABARAP no-stop

Plasmid

#123209

Purpose

Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1341

Plasmid

#119272

Purpose

control sgRNA in cells lacking rfp

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pChlr-7SE

Plasmid

#52168

Purpose

Encodes module 7 with amino acids 12S-16E for recognition of nt 2G

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 241-283 (PUM1 Human)

Expression

Bacterial

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 G116A

Plasmid

#123084

Purpose

Gateway entry clone encoding human GABARAPL1 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 116 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAMV:nAChRgamma-eGFP

Plasmid

#29693

Depositor

Henry Lester

Article

Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Insert

GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)

Tags

AMV promotor and poly(A) tail

Expression

Bacterial

Mutation

eGFP inserted after position S380

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) WT

Plasmid

#134690

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 ERbeta(1-255)

Plasmid

#35564

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

GST

Expression

Bacterial

Mutation

residues 1-255

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF_S214C

Plasmid

#51077

Purpose

mammalian expression of ARAF S214C

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

S214C

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

Plasmid

#127787

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32, Arg36 and Arg69 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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