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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 5981 - 6000 of 12282 results

  1. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137042-plasmid-map-sequence-id-275698

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  4. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid
    #123182
    Purpose
    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Publication
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    Use
    Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123182-plasmid-map-sequence-id-241413

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  6. pCEP4-HA-Flag-natT1R2 a22-end_S212N

    Plasmid
    #113951
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution
    Promoter
    CMV
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113951-plasmid-map-sequence-id-215164

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  8. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

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  9. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid
    #91733
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91733-plasmid-map-sequence-id-180936

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  10. FLAG-RFP-UCR1C

    Plasmid
    #67582
    Purpose
    Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells
    Depositor
    Graeme Carnegie
    Publication
    Wang et al Cell Signal. 2015 May;27(5):908-22. doi:
    Insert
    C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
    Tags
    FLAG and TagRFP
    Expression
    Mammalian
    Mutation
    Amino acid residues 80-136 of PDE4D3
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pYFP-ErbB2ΔC776

    Plasmid
    #66947
    Purpose
    Mammalian expression of rat ErbB2 ΔC776 tagged with YFP
    Depositor
    Mads Lerdrup, Bo van_Deurs
    Publication
    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ErbB2 (Erbb2 Rat)
    Tags
    6xHis, V5 Tag, and YFP
    Expression
    Mammalian
    Mutation
    Deletion of C776 (see depositor comment below on …
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry

    Plasmid
    #235096
    Purpose
    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain
    Depositor
    Mitchell Guttman
    Publication
    Bhat et al Nature. 2024 May;629(8014):1165-1173. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    Expression
    Mammalian
    Mutation
    The SRRM1 entry clone from DNASU was modified usi…
    Promoter
    CAG
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    235096-plasmid-map-sequence-id-470053

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  16. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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  18. pANAP-P2A-eRF(E55D)

    Plasmid
    #241290
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor
    Depositor
    Justin Taraska
    Publication
    Obashi et al Structure. Volume 34, Issue 1, 8 January
    Insert
    the amber suppressor tRNACUA-Leu
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV
    Available since
    Dec. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241290-plasmid-map-sequence-id-481082

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  19. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

    Plasmid
    #228726
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D56R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228726-plasmid-map-sequence-id-454011

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  20. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

    Plasmid
    #228725
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228725-plasmid-map-sequence-id-454009

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Showing: 5981 - 6000 of 12282 results