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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 5981 - 6000 of 14067 results

  5. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid
    #192443
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192443-plasmid-map-sequence-id-379076

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  7. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid
    #228733
    Purpose
    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228733-plasmid-map-sequence-id-453997

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  8. pmCherryC1 VAP-B KD/MD

    Plasmid
    #226410
    Purpose
    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAP-B (VAPB Human)
    Tags
    HA, T7-Xpress tag, and mCherry
    Expression
    Mammalian
    Mutation
    K87D/M89D mutant, unable to bind FFAT motifs
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    226410-plasmid-map-sequence-id-447859

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  9. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220612
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220612-plasmid-map-sequence-id-441117

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  10. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid
    #218863
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Publication
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8m
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    A25G F286Y
    Promoter
    hsp70
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218863-plasmid-map-sequence-id-434232

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  11. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid
    #218864
    Purpose
    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Publication
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8s
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    S26M F286Y Q315H
    Promoter
    hsp70
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218864-plasmid-map-sequence-id-433411

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  18. pLV_PP1-mCitrine

    Plasmid
    #172472
    Purpose
    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.
    Depositor
    Sean R Collins
    Publication
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    Use
    Lentiviral
    Tags
    mCitrine and prolactin signal sequence (cleaved p…
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172472-plasmid-map-sequence-id-342800

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  20. pcDNA3-Lyn-sACt-mCherry

    Plasmid
    #138216
    Purpose
    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-sACt-mCherry (Adcy10 Rat)
    Tags
    N-terminal targeting sequence from Lyn kinase and…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138216-plasmid-map-sequence-id-307426

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Showing: 5981 - 6000 of 14067 results