We narrowed to 59,538 results for: cloning

1. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKO (Hyg)

   Plasmid

   #110087

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Promoter

   Ptet

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-IRES2-GFP

   Plasmid

   #234875

   Purpose

   Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSYn

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS-21a-PcAad1

   Plasmid

   #163039

   Purpose

   expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

   Depositor

   Jean-Marie Francois

   Article

   Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

   Insert

   aryl-alcohol dehydrogenase
   

   Tags

   GST and His6

   Expression

   Bacterial

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCl-MCS_miniCMV_miRFP703

   Plasmid

   #134985

   Purpose

   The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   miRFP703
   

   Use

   Lentiviral

   Promoter

   minCMV

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGD289

   Plasmid

   #228113

   Purpose

   pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Kva1 retron, ncRNA with 90 bp rpoB donor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCPP5741

   Plasmid

   #128722

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

   Insert

   shcV-hopV1
   

   Expression

   Bacterial

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-NEK1_IDG-K

   Plasmid

   #189871

   Purpose

   Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NEK1 (NEK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCPP5910

    Plasmid

    #128717

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcO-hopO1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5058

    Plasmid

    #128710

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcF-hopF2
    

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCS108

    Plasmid

    #209329

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

    Plasmid

    #229387

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCD-Ugi22

    Plasmid

    #207004

    Purpose

    pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)

    Depositor

    Wayne Parrott

    Article

    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

    Insert

    Utricularia gibba insulator 22
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCD-Ugi21

    Plasmid

    #207005

    Purpose

    pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)

    Depositor

    Wayne Parrott

    Article

    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

    Insert

    Utricularia gibba insulator 21
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA type III TGF beta receptor (R3-OFF)

    Plasmid

    #10913

    Depositor

    Bob Weinberg

    Article

    Wang et al Cell. 1991 Nov 15. 67(4):797-805.

    Insert

    R3-OFF (Tgfbr3 Rat)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5/FRT/TO HSPA6

    Plasmid

    #19459

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA6 (HSPA6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

    Plasmid

    #189909

    Purpose

    Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ADCK5 (ADCK5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

    Plasmid

    #189885

    Purpose

    Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    COQ8A (COQ8A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pL0M-S-mNeonGreen-EC18153

    Plasmid

    #137075

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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