We narrowed to 59,538 results for: cloning

1. pL0M-S-tdTomato-EC18158

   Plasmid

   #137078

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

   Plasmid

   #170182

   Purpose

   Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   mCherry
   

   Tags

   3XFlag and T2A

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQTEV-RAB17

   Plasmid

   #34812

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   RAB17 (RAB17 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA(MS2)_zeo insert c-MYC

   Plasmid

   #164636

   Purpose

   sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

   Depositor

   Günter Schneider

   Insert

   target sequence for c-Myc activation (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN0604 (pDEST, HygR)

   Plasmid

   #210483

   Purpose

   pDEST vector with HygR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDN0603 (pDEST, NatR)

   Plasmid

   #210482

   Purpose

   pDEST vector with NatR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH51266

   Plasmid

   #50267

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-TagBFP-NS

   Plasmid

   #75342

   Purpose

   Multisite gateway entry clone for adding N-terminal fusions of TagBFP

   Depositor

   Rob Parton

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   TagBFP
   

   Expression

   Mammalian

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF CAG mc2 IRES neo

   Plasmid

   #206232

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCZGY2851

    Plasmid

    #87761

    Purpose

    Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG(Q103L)
    

    Tags

    PH

    Expression

    Worm

    Promoter

    Punc-17b

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAPi

    Plasmid

    #127547

    Purpose

    Gateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segment

    Depositor

    Luis Vidali

    Article

    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

    Type

    Empty backbone

    Use

    RNAi

    Promoter

    Ubiquitin

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p667-UBC-AKT1-V5-miniTurbo_IDG-K

    Plasmid

    #189889

    Purpose

    Gateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-miniTurbo-V5-AKT1_IDG-K

    Plasmid

    #189890

    Purpose

    Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICSL70008

    Plasmid

    #50336

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV-STX6

    Plasmid

    #31581

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    syntaxin 6 (STX6 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-EGFP-NS

    Plasmid

    #75341

    Purpose

    Multisite gateway entry clone for adding N-terminal fusions of EGFP

    Depositor

    Rob Parton

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUP202pol4

    Plasmid

    #67184

    Purpose

    Conjugative cloning vector

    Depositor

    Hauke Hennecke, Julia Vorholt

    Article

    Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

    Type

    Empty backbone

    Use

    Conjugative bacterial cloning vector

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO DNAJB3

    Plasmid

    #19471

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB3 (Dnajb3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. p663-UBC-miniTurbo-V5-CDK19_IDG-K

    Plasmid

    #158175

    Purpose

    Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK19 (CDK19 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pF hPGK mc2 IRES hygro

    Plasmid

    #206238

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge