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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,887 results for: Coli

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Showing: 6041 - 6060 of 9887 results

  2. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  3. pEXP5-NT/pT7 LasI

    Plasmid
    #193631
    Purpose
    Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasI
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193631-plasmid-map-sequence-id-382852

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  6. pEXP5-NT/pT7 LuxI

    Plasmid
    #193626
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxI
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193626-plasmid-map-sequence-id-382174

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  7. APRT

    Plasmid
    #64028
    Purpose
    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
    Depositor
    Vern Schramm
    Article
    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9
    Insert
    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    Tags
    Histidine x 6
    Expression
    Bacterial
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. zCD2

    Plasmid
    #122031
    Purpose
    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Zebrafish HDAC6 Catalytic Domain 2
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    122031-plasmid-map-sequence-id-240596

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  17. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

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Showing: 6041 - 6060 of 9887 results