We narrowed to 18,703 results for: Tec

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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YEp181-CUP1-His-Smt3

Plasmid

#99536

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLEXm-GST-ATG14

Plasmid

#99329

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

ATG14 (ATG14 Human)

Tags

GST-TEV tag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-MYRIP

Plasmid

#89586

Purpose

Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYRIP (Myrip Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Myosin

Plasmid

#79995

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LC-Myosin (Myl9 Mouse)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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hSRF-3xHA-IRES-hrGFP (#479)

Plasmid

#78347

Purpose

Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

Depositor

Matthew Alexander, Louis Kunkel

Insert

SRF (SRF Human)

Tags

3x HA tag and IRES-hrGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-dsRedM

Plasmid

#73592

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

dsRed

Expression

Mammalian

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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GPKA

Plasmid

#61091

Purpose

GFP-tagged cAMP-dependent PKA catalytic subunit

Depositor

Steven Green

Article

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Endosomes-13

Plasmid

#56433

Purpose

Localization: Endosomes, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits
ECFP-ER-0

Plasmid

#55342

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

ER (CALR Human)

Tags

ECFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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TPC2-G-GECO1.2

Plasmid

#207141

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET24_NusA-Tus

Plasmid

#165959

Purpose

Expression of soluble E. coli Tus protein

Depositor

Keith Pardee

Article

Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

Insert

Tus

Tags

His-NusA-Tev

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp15

Plasmid

#165120

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp15 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-mCherry-iLIDx6

Plasmid

#103782

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-mCherry-iLIDx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Mutant Human Cortactin-GFP

Plasmid

#71365

Purpose

mammalian expression of mutant human Cortactin-GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

cortactin (CTTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutation present at 72nd nucleotide

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only