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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-MYB-shRNA

Plasmid

#25790

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human MYB (MYB Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pBabe puro Myr HA PIK3CB

Plasmid

#12527

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele.

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 MP1 shRNA

Plasmid

#26632

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

MP1 shRNA (LAMTOR3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pBabe-Ypet-PBD

Plasmid

#24533

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Use

Retroviral

Tags

Ypet

Expression

Mammalian

Available Since

Aug. 19, 2010

Availability

Academic Institutions and Nonprofits only
p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Dot1L_1

Plasmid

#36339

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Dot1L (DOT1L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
MSCV P2Gm p53

Plasmid

#19751

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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p6614 MSCV-IP N-HAonly PXMP3

Plasmid

#35012

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PXMP3 (PEX2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-SSeCKs T751A

Plasmid

#107142

Purpose

full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs T751A (Akap12 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed threonine 751 to alanine

Promoter

gag

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig1

Plasmid

#64841

Purpose

A lentiviral vector for the expression of mouse Olig1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig1 (Olig1 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP-SL08

Plasmid

#218247

Purpose

1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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TRAF6mut-UTR

Plasmid

#15094

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

TRAF6 3'UTR (TRAF6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pMJK2243

Plasmid

#227688

Purpose

Empty lentivector to overexpress gene of interest

Depositor

Curt Civin

Article

Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgMTHFD2_1

Plasmid

#106313

Purpose

Express Cas9 and sgRNA targeting MTHFD2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2 (MTHFD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V Y40C

Plasmid

#12276

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1-dUBA

Plasmid

#74205

Purpose

Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed aa 877 to encode for premature stop codon

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only