We narrowed to 10,133 results for: ADA

1. pD40-His/V5-c-Myc

   Plasmid

   #45597

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc (Myc Mouse)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-cFos-XRI-V5

   Plasmid

   #178058

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-V5
   

   Use

   AAV

   Tags

   V5-MBP

   Expression

   Mammalian

   Promoter

   Mouse c-fos promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVH-EF1a-GFP-P2A-OCT4

   Plasmid

   #130692

   Purpose

   Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXJ42-p200 CUX1

   Plasmid

   #100813

   Purpose

   Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

   Depositor

   Alain Nepveu

   Article

   Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

   Insert

   p200 CUX1 (CUX1 Human)
   

   Use

   Note that this plasmid does not have an sv40 ori

   Tags

   HA and Myc

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dCas12a-ABE7

   Plasmid

   #193644

   Purpose

   Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA-TadA*7.10, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-neo-PP1-Alpha-H248K

   Plasmid

   #171268

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PPP1CA-His248Lys (H248K)

   Promoter

   CMV

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Solo-mStayGold-CENPA

   Plasmid

   #227283

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCh-BICD2*-strep

   Plasmid

   #120168

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   BICD cargo adaptor 2 (Bicd2 Mouse)
   

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated BICD2: 15-595 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB-hCRY1-mScarlet-I-CD4-bla

   Plasmid

   #179442

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

    Plasmid

    #101064

    Purpose

    AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2_L398T
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSD138A

    Plasmid

    #218130

    Purpose

    Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    luxAB
    

    Tags

    scP22

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC_hfnCas12a_CRATES

    Plasmid

    #176251

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Integrin beta5-2XEGFP

    Plasmid

    #139779

    Purpose

    Detecting Integrin beta5 by fluorescence microscopy

    Depositor

    Staffan Stromblad

    Article

    Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

    Insert

    Integrin subunit beta5 (ITGB5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

    Plasmid

    #198394

    Purpose

    eRF1 (AAQ mutant) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    eRF1 (ETF1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    mutated GGQ motif (G183/184A)

    Promoter

    CMV

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-EMTB-TurboID-V5

    Plasmid

    #190736

    Purpose

    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    ensconsin (MAP7 Human)
    

    Tags

    Myc, TurboID, and V5

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

    Plasmid

    #178528

    Purpose

    Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    rtTA, SspB-mScarlet-I-MYPT (1-169aa)
    

    Expression

    Mammalian

    Promoter

    CAG, Tet responsive element

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

    Plasmid

    #171150

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_SST1.0

    Plasmid

    #208665

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_eA3A T31A-NG-nCas9

    Plasmid

    #163539

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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