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pOPINO_C5

Plasmid

#171925

Purpose

Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_C5

Tags

His6

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSinREP5-iAchSnFR

Plasmid

#140643

Purpose

Acetylcholine sensor for sindbis virus

Depositor

J. Julius Zhu

Article

Borden et al bioRxiv 2020

Insert

Ach sensor V9

Expression

Mammalian

Promoter

SP6

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX

Plasmid

#138387

Purpose

Empty RMCE vector (phiC31 attB/loxP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-RNb

Plasmid

#128787

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-RNb

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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ssGNb-mCherry

Plasmid

#128788

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

ssGNb-mCherry

Expression

Mammalian

Available Since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pN40

Plasmid

#128898

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab2AQL-BirA*-HA-MAO (RAB2A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I3R

Plasmid

#125065

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I3L

Plasmid

#125032

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG024

Plasmid

#113991

Purpose

Level 1 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ MBIC5-mCer3

Plasmid

#74116

Purpose

For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

Depositor

Michael Tsang

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Transgenic construct

Tags

mCerulean 3

Promoter

Myl7 (Cmlc2)

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120033127

Plasmid

#46011

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120033127 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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EcoHIV-mScarlet

Plasmid

#235598

Purpose

EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

Depositor

Michael Shtutman

Article

Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

Insert

NL4-3 EcoHIV mScarlet

Use

Lentiviral

Tags

mScarlet

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-20nmEML-mRFP

Plasmid

#231181

Purpose

Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vector

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP

Plasmid

#221481

Purpose

GFP lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA-UGI

Plasmid

#221220

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSST-0.3k-Venus-WPRE

Plasmid

#156393

Purpose

Expresses Venus under SST gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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