We narrowed to 8,732 results for: Set

1. pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)

   Plasmid

   #167357

   Purpose

   Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t

   Depositor

   Michael Jensen

   Article

   Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

   Insert

   TDH3p:his6-mKate2-LipPks1-IDP1t
   

   Use

   Unspecified

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR_full-site

   Plasmid

   #186277

   Purpose

   Reporter plasmid for full-site selection assay

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pATF4-donor

   Plasmid

   #113805

   Purpose

   CRISPR donor plasmid to tag human transcription factor ATF4 with GFP

   Depositor

   Kevin White

   Insert

   ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
   

   Use

   CRISPR

   Mutation

   rs140428747

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAP72_MS2

   Plasmid

   #220623

   Purpose

   Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Type

   Empty backbone

   Use

   Shuttle vector

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-eSIBR

   Plasmid

   #71665

   Purpose

   intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

   Depositor

   Philip Washbourne

   Article

   Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

   Insert

   intronic eSIBR cassette
   

   Use

   RNAi; Multi-site gateway

   Promoter

   None

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)

   Plasmid

   #167356

   Purpose

   Integrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1t

   Depositor

   Michael Jensen

   Article

   Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

   Insert

   TDH3p:his6-mKate2-MBP-IDP1t
   

   Use

   Unspecified

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdx1-mTQ2-P2A-FlpO-PA

   Plasmid

   #67279

   Purpose

   Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   mTQ2=mturquoise2 blue fluorescent gene
   FlpO recombinase
   

   Tags

   linked to FlpO through an P2A ribosomal skipping …

   Expression

   Mammalian

   Promoter

   pdx

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CasRX_Pac

   Plasmid

   #194803

   Purpose

   CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

   Depositor

   Alexander Paredez

   Article

   Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

   Insert

   CasRX
   

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTC201B

    Plasmid

    #52193

    Purpose

    for creating reversible targeting construct

    Depositor

    Tian Chi

    Article

    Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

    Insert

    promoter-trap cassette Neo-Ires-GFP
    

    Use

    Cre/Lox

    Tags

    Neo, GFP

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3_alpha1-AtUBQ10:GFP-T35S

    Plasmid

    #211836

    Purpose

    Plant transient expression cassette for GFP (control) under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    GFP control
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Promoter

    UBIQUITIN10

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB01

    Plasmid

    #111957

    Purpose

    Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Insert

    RFP
    

    Tags

    HaloTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LT0

    Plasmid

    #210551

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT0::mCherrySSM
    

    Expression

    Plant

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LT1

    Plasmid

    #210552

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT1::mCherrySSM
    

    Expression

    Plant

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LT2

    Plasmid

    #210553

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT2::mCherrySSM
    

    Expression

    Plant

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonorTp

    Plasmid

    #211788

    Purpose

    pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

    Depositor

    Silvia Cardona

    Article

    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

    Insert

    dhfr
    

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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