We narrowed to 11,759 results for: 110

1. pLKO_FLAG.V5.C_NGFR

   Plasmid

   #158259

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG.V5.C

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_HA.AU1.NWS_NGFR

   Plasmid

   #158234

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.AU1.NWS

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_HA.AU1.FLAG_NGFR

   Plasmid

   #158236

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.AU1.FLAG

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_NWS.AU1.V5_NGFR

   Plasmid

   #158237

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.AU1.V5

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-FOXE1

   Plasmid

   #101439

   Purpose

   Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   FOXE1 (FOXE1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

   Plasmid

   #136657

   Purpose

   Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:LMG18311 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

   Plasmid

   #136654

   Purpose

   Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:CNRZ1066 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Ataxin-1, 11NQ (Mouse) [N76/8R]

   Plasmid

   #128641

   Purpose

   Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/8.

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Ataxin-1, 11NQ (Mouse) recombinant mouse monoclonal antibody (Atxn1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-puro-shRPS6

   Plasmid

   #125780

   Purpose

   constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   shRPS6 (RPS6 Human)
   

   Use

   RNAi

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_BRD003-sgCh2-2

    Plasmid

    #125767

    Purpose

    constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgCh2-2
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_BRD003-sgCh2-4

    Plasmid

    #125768

    Purpose

    constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgCh2-4
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-RAF1-H466R

    Plasmid

    #116653

    Purpose

    Lentiviral expression of RAF1 H466R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Mutation

    H466R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-RAF1-L86R

    Plasmid

    #116655

    Purpose

    Lentiviral expression of RAF1 L86R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Mutation

    L86R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-RAF1-N64K

    Plasmid

    #116657

    Purpose

    Lentiviral expression of RAF1 N64K

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Mutation

    N64K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-RAF1-Q422R

    Plasmid

    #116658

    Purpose

    Lentiviral expression of RAF1 Q422R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Mutation

    Q422R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-RAF1-R164Q

    Plasmid

    #116659

    Purpose

    Lentiviral expression of RAF1 R164Q

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Mutation

    R164Q

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-RAF1-V537I

    Plasmid

    #116661

    Purpose

    Lentiviral expression of RAF1 V537I

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Mutation

    V537I

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid

    #118261

    Purpose

    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid

    #118259

    Purpose

    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid

    #118258

    Purpose

    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge