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pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G12-E

Plasmid

#25761

Purpose

Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Gateway Cloning

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (W40A)

Plasmid

#252263

Purpose

Retroviral expression of EGFP-mouse Fis1 (W40A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (W40A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating tryptophan 40 to alanine (W40A) in Fis1 …

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_AA535-889

Plasmid

#240323

Purpose

Entry vector for Gateway with USP11 (AA535-889)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

AA535-889

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_AA535-776

Plasmid

#240322

Purpose

Entry vector for Gateway with USP10 (AA535-776)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

AA535-776

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_AA535-1102

Plasmid

#240324

Purpose

Entry vector for Gateway with USP12 (AA535-1102)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

AA535-1102

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7

Plasmid

#240319

Purpose

Entry vector for Gateway with USP7

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_AA886-1102

Plasmid

#240326

Purpose

Entry vector for Gateway with USP14 (AA886-1102)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

AA886-1102

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201_PARP1_del119/120

Plasmid

#240318

Purpose

Entry vector for Gateway with PARP1 (AA119/120 deletion)

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARP1 (PARP1 Human)

Expression

Bacterial

Mutation

AA119/120 was deleted

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_AA1-208

Plasmid

#240321

Purpose

Entry vector for Gateway with USP9 (AA1-208)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

AA1-208

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_C223S

Plasmid

#240320

Purpose

Entry vector for Gateway with USP8 (C223A)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

C223A

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_USP7_AA776-1102

Plasmid

#240325

Purpose

Entry vector for Gateway with USP13 (AA776-1102)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

AA776-1102

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-SV40-pA (JDW 1417)

Plasmid

#242570

Purpose

Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCherry stop SV40 pA

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway Cloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-ABL2(K281M)

Plasmid

#215468

Purpose

Gateway entry vector with Arg kinase-dead (K281M)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Gateway Cloning

Mutation

K281M (kinase-inactivating)

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAD131_hIrg1_M154I_pCMV6

Plasmid

#239120

Purpose

hACOD1 with M154I mutation in mammalian expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

Myc and FLAG

Expression

Mammalian

Mutation

M154I

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_CG6867_nostop

Plasmid

#240238

Purpose

Gateway entry clone with CG6867 without stop codon

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN11

Plasmid

#215476

Purpose

Gateway entry vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway Cloning

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12(D199A,C231S)-Clover

Plasmid

#215522

Purpose

Gateway entry vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway Cloning

Tags

Clover

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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