We narrowed to 65,197 results for: Eng

1. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

   Plasmid

   #170829

   Purpose

   PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d CLCN3 gRNA2
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

   Plasmid

   #170822

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA1
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265E7

   Plasmid

   #213470

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cdd-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265E8

   Plasmid

   #213471

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-syn-FLEX-tTA

   Plasmid

   #178516

   Purpose

   AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 mePOU-POU

   Plasmid

   #206392

   Purpose

   Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   ePOU
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. iCAP-BI-49-KanR

   Plasmid

   #203534

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI49 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. iCAP-BI-65-KanR

   Plasmid

   #203536

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI65 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet1-LanCL2-H264A-C-Ter-His

    Plasmid

    #154187

    Purpose

    To express LanCL2-H264A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His264->Ala264

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-15b-LanCL1-H219A

    Plasmid

    #154190

    Purpose

    To express LanCL1-H219A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His219->Ala219

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-15b-LanCL1-C322A

    Plasmid

    #154191

    Purpose

    To express LanCL1-C322A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys322->Ala322

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-p2x-LanCL2

    Plasmid

    #154192

    Purpose

    To express MBP-LanCL2 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Tags

    Maltose binding protein (MBP)

    Expression

    Bacterial

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-TTBG

    Plasmid

    #115232

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
    

    Expression

    Mammalian

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-TTBL

    Plasmid

    #115233

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
    

    Expression

    Mammalian

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLKO.1-Cd155 shRNA2

    Plasmid

    #166488

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLKO.1-Cd155 shRNA1

    Plasmid

    #166487

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-N1-MOB1

    Plasmid

    #166477

    Purpose

    Expresses fusion of mCherry and MOB1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, MOB1 (MOB1A Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PatoM-OG241

    Plasmid

    #72842

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoM
    

    Expression

    Bacterial

    Promoter

    PatoM

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEXP5-NT-7th R

    Plasmid

    #42573

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated calmodulin gene
    

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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