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Plasmid#171295PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPtRNA[Met] P. rubens Pol-III promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK022
Plasmid#171294PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPu6 hom., P. rubens Pol-III promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK021
Plasmid#171293PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPu3 hom., Putp25, P. rubens Pol-III promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK085
Plasmid#171357PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Leu] P. rubens Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK083
Plasmid#171355PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTu6 hom., P. rubens Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK082
Plasmid#171354PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTu3 hom., Tutp25, P. rubens Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-10X-tRNA
Plasmid#70123PurposeGateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAsDepositorInsert10X-tRNA block
UseTagsExpressionMutationPromoterNone – SUP4 pol III promoter provided by insertAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p8.91
Plasmid#187441PurposeLentiviral packaging plasmid expressing gag and pol.DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLVgag-3NES-Cre
Plasmid#207252PurposeGeneration of CreVLPs when used in conjunction with MMLV gag-pol during productionDepositorInsertMMLVgag-3NES-Cre
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCT-tRNA
Plasmid#133813PurposeCRISPR-Cas9 system for genetic manipulation of Candida tropicalisDepositorInsertcassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
Plasmid#126552PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsCD8a and GFPExpressionMutationDelta gag/polPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PU6::unc-119_sgRNA
Plasmid#46169Purposeunc-119 targeting sgRNADepositorInsertunc-119 targeting sgRNA (unc-119 Synthetic)
UseCRISPRTagsExpressionMutationPromoterC. elegans U6 snRNA pol III promoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH*deltavpu/RT-
Plasmid#136985PurposeSecond-generation lentiviral packaging plasmid lacking reverse transcriptase activityDepositorInsertHIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
UseTagsExpressionMammalianMutationcarries D110E mutation in the reverse transcripta…PromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK087
Plasmid#171359PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shGAC
Plasmid#110410PurposeshGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.DepositorInsertGLS glutaminase (GLS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CTE-Luc
Plasmid#60881PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTEDepositorInsertLuc-CTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG424-10X-tRNA
Plasmid#70125PurposeYeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae intronsDepositorInsert10X-tRNA block
UseTagsExpressionYeastMutationPromoterNone – SUP4 pol III promoter provided by insertAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
gB gag LV
Plasmid#12328DepositorInsertgag/pol
UseLentiviralTagsExpressionMammalianMutationThe CypA binding region of the WT gag was replace…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
Watermelon
Pooled Library#155257PurposeLineage tracing libraryDepositorExpressionMammalianUseLentiviralAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPR Poison Exon Knockout Library
Pooled Library#138084PurposeThe human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JS200 strain
Bacterial Strain#11794DepositorBacterial ResistanceNoneAvailable sinceJune 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
Barcode Library 0 (BCL0)
Pooled Library#179851PurposeLentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.DepositorExpressionMammalianUseLentiviralAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Barcode Library 1 (BCL1)
Pooled Library#179852PurposeLentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.DepositorExpressionMammalianUseLentiviralAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse genome-wide lentiviral CRISPR gRNA library version 2
Pooled Library#67988PurposeMouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human genome-wide lentiviral CRISPR gRNA library version 1
Pooled Library#67989PurposeHuman genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEV53D
Plasmid#44168DepositorInsertEquine infectious anemia virus (EIAV) gag-pol,rev, and S2
UseLentiviralTagsExpressionMammalianMutationA131T mutation in gag; Deletion of viral LTR sequ…PromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only