We narrowed to 8,922 results for: Pol

1. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

   Plasmid

   #159496

   Purpose

   Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

   Plasmid

   #159495

   Purpose

   Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

   Plasmid

   #121748

   Purpose

   pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   OLLAS epitope tag-IRES2-eGFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IX503: pMVP (L3-L2) OLLAS epitope tag + polyA

   Plasmid

   #121752

   Purpose

   pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   OLLAS epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. IX401: pMVP (L3-L2) 6x His epitope tag + polyA

   Plasmid

   #121754

   Purpose

   pMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   6x His epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIp128-P30-His-pol30(K127/164R)

   Plasmid

   #99545

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K127R, K164R

   Promoter

   POL30

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

   Plasmid

   #107270

   Purpose

   Expresses Cas9 upon induction with doxicycline.

   Depositor

   Pablo Menéndez

   Article

   Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

   Inserts

   hSpCas9
   rtTA
   bGH polyA-SV40polyA
   

   Use

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

   Plasmid

   #216320

   Purpose

   Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Luciferase + splice donor site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

   Plasmid

   #84603

   Purpose

   Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

   Depositor

   James Chen

   Article

   Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

   Insert

   mCherry
   

   Use

   Zebrafish transgenesis

   Tags

   ODC1 destabilizing element

   Expression

   Mutation

   Promoter

   8xGliBS-delta-crystallin minimal promoter

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

    Plasmid

    #89459

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

    Plasmid

    #174028

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

    Plasmid

    #174029

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-mScarlet
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

    Plasmid

    #180486

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    VARNAM A122D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

    Plasmid

    #46835

    Purpose

    Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-CMV

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid

    #208972

    Purpose

    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-EGFPnls barcode-206-SV40 polyA

    Plasmid

    #190875

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode206
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

    Plasmid

    #46836

    Purpose

    Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

    Plasmid

    #177144

    Purpose

    Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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