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Plasmid#79871PurposeFor generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorInsertGFP-GFE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR-Hygro
Plasmid#176063PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertLivePAR
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Zeo
Plasmid#227268PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-LivePARBackbone
Plasmid#176526PurposeExpression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePARDepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CSII-prEF1a-mCherry-3xNLS
Plasmid#125262PurposeFluorescent reporter for cell sizeDepositorInsertmCherry-3xNLS
UseLentiviralTags3xNLSExpressionMammalianMutationPromoterEF1aAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mGreenLantern
Plasmid#220596PurposeConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.DepositorInsertmGreenLantern
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF0432 Ef1a-Dn29-2a-EGFP
Plasmid#193459PurposeExpression of Dn29 RecombinaseDepositorInsertDn29
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP (no signal control)
Plasmid#170167PurposeMammalian Expression of no signal controlDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationn/aPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-WT_ABCA3-GFP
Plasmid#188548PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion proteinDepositorInsertABCA3:GFP (ABCA3 Human)
UseLentiviralTagsGFPExpressionMutationPromoterEF1aLAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8f-WPRE
Plasmid#176756PurposeAAV-mediated expression of GCaMP8f under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry
Plasmid#114471PurposeFlp-dependent mCherry ControlDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-EGFP-WPRE-pA
Plasmid#37085PurposeExpresses EGFP in cells lacking Cre (Cre-Off)DepositorInsertEGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
CSII-prEF1a-E2-Crimson-NLS
Plasmid#125263PurposeFluorescent reporter for cell sizeDepositorInsertE2-Crimson-NLS
UseLentiviralTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.28.BB.3z
Plasmid#200680PurposeModular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-eGFP
Plasmid#191104PurposeLentiviral expression plasmid of hMLH1dn with P2A-eGFP reporterDepositorInserthMLH1dn
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-Hygro
Plasmid#195512PurposeLentiviral expression plasmid of hMLH1dn with P2A-HygroR markerDepositorInserthMLH1dn-P2A-HygroR (MLH1 Human)
UseLentiviralTagsP2AExpressionMammalianMutationHuman MLH1 del754-756PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAID-EF1a linearizing in pX330
Plasmid#140610PurposeCrispr/Cas9 plasmid for linearization of pAID-EF1aDepositorInsertsgRNA for pAID-EF1a plasmid digestion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ABCD3-RFP-Puro
Plasmid#170168PurposeMammalian Expression of Ef1a-ABCD3-RFP-PuroDepositorInsertABCD3-RFP-Puro
UseTagsExpressionMammalianMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-10xGCN4_Hygro
Plasmid#192651Purpose3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance markerDepositorInsertdCas9-10xGCN4
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only