We narrowed to 7,391 results for: ef1a;

1. AAV Ef1a-EGFP-WPRE

   Plasmid

   #135428

   Purpose

   Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence

   Depositor

   Rylan Larsen

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-AAVS1-Ef1a-Blast

   Plasmid

   #227267

   Purpose

   Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-Ef1a-SOX2-3xFLAG

   Plasmid

   #216197

   Purpose

   Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-KLF2-T2A-mCHERRY

   Plasmid

   #224295

   Purpose

   Expresses mouse KLF2 and mCherry under EF1a promoter

   Depositor

   Nikhil Joshi

   Article

   Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

   Insert

   KLF2 T2A mCherry (Klf2 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

   Plasmid

   #229545

   Purpose

   Lentiviral construct to express rtTA and SKM proteins

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hEF1a

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Expression

   Mammalian

   Promoter

   U3MSCV

   Available Since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-fDIO-Cre

    Plasmid

    #121675

    Purpose

    Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    NLS-CRE-HA
    

    Use

    AAV

    Tags

    3xHA and SV40-NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-H2B-miRFP670

    Plasmid

    #212668

    Purpose

    Express tagged H2B

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    miRFP670

    Promoter

    EF-1a

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-EF1a-MegaGate-DD-Hygro

    Plasmid

    #175267

    Purpose

    MegaGate destination with C-terminal DD tag and hygromycin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Tags

    Destabilization Domain

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-Ef1a-InteinC-CreC

    Plasmid

    #187615

    Purpose

    AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    InteinC-CreC
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-EF1alpha-dCas9-p300_Blast

    Plasmid

    #192653

    Purpose

    3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Ef1a-CreN-InteinN

    Plasmid

    #187614

    Purpose

    AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    CreN-InteinN
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLENTI EF1alpha FLAG/HA-PALI1

    Plasmid

    #114445

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIDFA-EF1a-NmScarlet-mAID

    Plasmid

    #140615

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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