We narrowed to 680 results for: intein

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pTXB1-APEX2-F

Plasmid

#160083

Purpose

Bacterial expression of C-terminally FLAG-tagged APEX2 peroxidase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

APEX2 peroxidase

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max C-terminal

Plasmid

#137172

Purpose

Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_C-terminal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax C-terminal

Plasmid

#137174

Purpose

Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_C-terminal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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H6SUMO-IntC-P53(304-393)

Plasmid

#200316

Purpose

bacterial expression of P53 TET-CTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6-SUMO and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53(1-303)IntN

Plasmid

#200312

Purpose

Bacterial expression of P53 NTAD-DBD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Tags

His6GB1tev and designed intein N

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP4

Plasmid

#160087

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP1

Plasmid

#160084

Purpose

Bacterial expression of Tn5-APEX2 fusion protein, no linker

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP2

Plasmid

#160085

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
H6GB1tev-IntC-P53(304-393)

Plasmid

#200315

Purpose

bacterial expression of P53 TETCTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6GB1tev and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pWP172

Plasmid

#198796

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP170

Plasmid

#198792

Purpose

Co-expression plasmid for the FLP, PVD neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP171

Plasmid

#198795

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Peat4prom9-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pWP101

Plasmid

#198791

Purpose

Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A K31A

Plasmid

#105696

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 and 31 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTYB-pLAC-F112S

Plasmid

#48727

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Phenylalanine 12 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-L108S

Plasmid

#48725

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Leucine 108 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I98S

Plasmid

#48723

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 98 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I73S

Plasmid

#48722

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 73 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-V69S

Plasmid

#48721

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Valine 69 to Serine

Promoter

T7

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only