We narrowed to 2,731 results for: nags

1. Carl-CeNL-KDEL_pcDNA3

   Plasmid

   #89509

   Purpose

   Cyan color variant of bright luminescent protein, targeted to the endoplasmic reticulum

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GLUT9-eGFP/pcDNA-DEST47

   Plasmid

   #18730

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted TAA

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

3. LAMP-OeNL_pcDNA3

   Plasmid

   #89525

   Purpose

   Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nano-lantern(cAMP-3.3)/pcDNA3

   Plasmid

   #53595

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Nano-lantern(Ca2+)_150/pcDNA3

   Plasmid

   #51984

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Nano-lantern(Ca2+)_11/pcDNA3

   Plasmid

   #51987

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-4GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Nano-lantern(cAMP-0.4)/pcDNA3

   Plasmid

   #53593

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pJYDN3p

   Plasmid

   #162457

   Purpose

   Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nano-lantern-mitochondria/pcDNA3

   Plasmid

   #51973

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET29b-dspB(E184Q)-6xHis

    Plasmid

    #175802

    Purpose

    Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEU3-NII-GLICNot-C-His

    Plasmid

    #140187

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    6xHis and GST

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS403red-­luxTA2-­4F

    Plasmid

    #166030

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS405red-­phlTA2-­1E

    Plasmid

    #166029

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGK415-­luxTA2-­4F

    Plasmid

    #165971

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEU3-NII-FLAGLICNot

    Plasmid

    #140184

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Yellow Cameleon-Nano30/pcDNA3

    Plasmid

    #51963

    Purpose

    mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111926

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge