Addgene

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pGEX Eat2-SH2

Plasmid

#46423

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Eat2 (SH2D1B Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-132

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-2T SHP1 deltaP

Plasmid

#8596

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deltaP (deletion of phosphatase domain, amino aci…

Promoter

Available since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Brk-SH2

Plasmid

#46411

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Brk (PTK6 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 74-176

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDF1-ERBB4 (E872K)

Plasmid

#29543

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pET22b-ecDHFR-Y100F

Plasmid

#110826

Purpose

Bacterial expression of E.coli DHFR Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

Y100 mutated to F100

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX SHF-SH2

Plasmid

#46491

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHF (SHF Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 317-423

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHE-SH2

Plasmid

#46490

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHE

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 382-492

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHD-SH2

Plasmid

#46489

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHD (SHD Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 227-340

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Rin3-SH2

Plasmid

#46476

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Rin3 (RIN3 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 55-157

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-ERBB4 (E317K)

Plasmid

#29537

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E452K)

Plasmid

#29538

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pGEX Rin2-SH2

Plasmid

#46475

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Rin2 (RIN2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 94-193

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Nap4-SH2

Plasmid

#46456

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nap4 (SOCS7 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 395-514

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Nsp1-SH2

Plasmid

#46459

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp1 (SH2D3A Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 3-119

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-ERBB4 (E563K)

Plasmid

#29541

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5 713

Plasmid

#65246

Purpose

Expression of HA tagged ERK5 AA 2-713 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 713 (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 714-817

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (R544W)

Plasmid

#29540

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pT7-TaGu-RTD-3xF-ORF

Plasmid

#238490

Purpose

For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Expression

Mutation

DD660/661->AA

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-TaGu-END-3xF-ORF

Plasmid

#238489

Purpose

For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Expression

Mutation

DD1057/1070->AA

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge