We narrowed to 18,589 results for: MUT

1. pET30Xa-SBL1(160S)

   Plasmid

   #66872

   Purpose

   Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
   

   Tags

   Histag from pET30Xa-LIC

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7lac

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWp63-clone32 (Opti-SpCas9)

   Plasmid

   #131736

   Purpose

   Expresses Opti-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   Opti-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   R661A+K1003H

   Promoter

   EFS

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSC43

   Plasmid

   #104817

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

   Plasmid

   #127787

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32, Arg36 and Arg69 to Ala

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5-rat ERK2-D319N

   Plasmid

   #40820

   Depositor

   Natalie Ahn

   Article

   Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

   Insert

   MAPK1 (Mapk1 Rat)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   D319N

   Promoter

   CMV

   Available Since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-MtPylRS (human-opti)

   Plasmid

   #164080

   Purpose

   To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtPylRS-478(human-opti)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-3xFLAG-Puro PAK5 K478R K469R

   Plasmid

   #167180

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R K469R
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP-pk-H302A

   Plasmid

   #115618

   Purpose

   Expresses human Parkin H302A in mammalian cells

   Depositor

   Edward Fon

   Article

   Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

   Insert

   Parkin (PRKN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H302A

   Promoter

   CMV

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM4889 (pCV0001)

    Plasmid

    #120079

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Mutation

    mobA Y25F

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVsc.U6-sgFah.CMV/CB-EGFP

    Plasmid

    #121508

    Purpose

    Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Q842gp120-8his

    Plasmid

    #100932

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-PRMT9 L182A/D183A/I184A/G185A

    Plasmid

    #79676

    Purpose

    mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L182A/D183A/I184A/G185A catalytic mutant

    Promoter

    CMV

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. 8200 Bicistronic_GFP_ires_hygro

    Plasmid

    #64375

    Purpose

    This a retroviral expression plasmid expressing GFP along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    EGFP

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMyc2ElbLuc,

    Plasmid

    #53244

    Purpose

    reporter plasmid containing 2 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc2ElbLuc (MYC Human)
    

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. R9-WT

    Plasmid

    #125210

    Purpose

    Expressing wildtype human Rad9b gene

    Depositor

    Richard Finnell

    Article

    Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

    Insert

    Rad9b (RAD9B Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-ERBB4 (E872K)

    Plasmid

    #29535

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Expression

    Mammalian

    Mutation

    changed glutamic acid 872 to lysine

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only