We narrowed to 14,429 results for: nts

1. pEPOR0SP0011

   Plasmid

   #117530

   Purpose

   Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-xCas9 3.7 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSP64R1 SHP2 E76A

   Plasmid

   #8327

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   E76A (activated mutant)

   Promoter

   Available since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

3. KHBD00549

   Plasmid

   #39644

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3340 (Kr Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TDP43 NOTAG11

   Plasmid

   #28210

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa193-274 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

   Plasmid

   #68280

   Purpose

   SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Mkk2DS NLS mCherry (MKK2 Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   RPS2

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. DCT.VV.Orf110

   Plasmid

   #12798

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf110
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

7. KHBD00175

   Plasmid

   #34328

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5187 (Doc2 Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

8. KHBD00140

   Plasmid

   #34294

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5093 (Doc3 Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMOD_A1010

   Plasmid

   #91030

   Purpose

   Module A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   ZmUbi

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A1002

    Plasmid

    #91029

    Purpose

    Module A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    AtUbi10

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTaCas9-H840A_5

    Plasmid

    #91172

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTaCas9-D10A_6

    Plasmid

    #91171

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAtCas9-D10A_3

    Plasmid

    #91161

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOD_A1410

    Plasmid

    #91035

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    ZmUbi

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTC440

    Plasmid

    #91225

    Purpose

    protoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting barley MLO
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTC441

    Plasmid

    #91218

    Purpose

    protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting MLO gene in barley
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTC436

    Plasmid

    #91222

    Purpose

    protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting barley MLO
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTC437

    Plasmid

    #91219

    Purpose

    protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting MLO gene in barley
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAH750

    Plasmid

    #91212

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmalc4x_V426F_TEV

    Plasmid

    #47702

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with V426F mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed V426F

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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