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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PJ23102R1LuxR_PCTR1E2Crimson

Plasmid

#250892

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic promoter; component of BNeuAS6; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PCTR1EGFP_PJ23102R1LuxR

Plasmid

#250885

Purpose

EGFP expressed under PCT synthetic promoter and LuxR expressed under BBa_J23102 promoter; component of BNeuAS3; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

EGFP
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux_R1_CI*

Plasmid

#250883

Purpose

Expresses frame-shifted cI under the control of PLux promoter; part of BNeuAS2 and BNeuAS3; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame-shifted cI protein

Promoter

Plux

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVS-RT-pDGB3alpha2

Plasmid

#240730

Purpose

BCTV vector containing the GUS:NPTII repari template. Lacks the BCTV virion sense ORFs.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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BCTV-VS domesticated-pUPD2

Plasmid

#240748

Purpose

Domesticated BCTV virion sense ORFs with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Mutation

2nt substitution in V3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-pUPD2

Plasmid

#240715

Purpose

BCTV complementary sense ORFs with mutated Rep with GoldenBraid C1 grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTV-VS WT-pUPD2

Plasmid

#240716

Purpose

WT BCTV virion sense ORFs prior to domestication for GoldenGate cloning with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVS-YPET-pDGB3alpha1

Plasmid

#240723

Purpose

BCTV vector for YPET expression. Lacks the BCTV virion sense ORFs.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

YPET

Expression

Plant

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-YPET-pDGB3alpha1

Plasmid

#240725

Purpose

BCTV vector for YPET expression. Rep has been mutated.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

YPET

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-RT-pDGB3alpha1

Plasmid

#240728

Purpose

BCTV vector containing the GUS:NPTII repair template for HDR experiments. Rep has been mutated.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_HyPB_RFP

Plasmid

#248201

Purpose

HyPB RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_Antgra4_RFP

Plasmid

#248203

Purpose

Antgra4 RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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LentiX_(203-bp-DIAL-YB_TATA)-mGL-BGH_pKG2945

Plasmid

#246342

Purpose

203-bp DIAL Reporter Lentivirus expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Lentiviral and Synthetic Biology

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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excision_reporter_HyPB

Plasmid

#248200

Purpose

HyPB and synthetic transposase excision reporter and targeted integration reporter

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

Excision RFP reporter

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Article

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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FSW FLAG

Plasmid

#246725

Purpose

Neuron-specific expression of insert with a FLAG tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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FSW mKeima

Plasmid

#246726

Purpose

Neuron-specific expression of insert with a mKeima tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

mKeima

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti mKeima

Plasmid

#246746

Purpose

Mammalian and lentiviral expression of insert with a mKeima tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

mKeima

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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