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pME-H2B-mScarlet3-S2 (JDW 1514)

Plasmid

#242552

Purpose

Gateway middle entry clone containing H2B-mScarlet3-S2; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-S2

Use

Gateway Cloning

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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RAM-d2TTA-TRE-hM4Di-mCherry-WPREpA

Plasmid

#223670

Purpose

AAV expression of hM4Di from RAM promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

hM4Di

Use

AAV

Tags

mCherry

Promoter

pRAM and TRE

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mScarlet-3H (JDW 1513)

Plasmid

#242551

Purpose

Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-H

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Sema4D-Flag

Plasmid

#190646

Purpose

Expresses Mouse Sema4D with C Terminal Flag Tag

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4d (Sema4d Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-mSema4D

Plasmid

#190645

Purpose

Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4d (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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HA-mNM2A

Plasmid

#236531

Purpose

For expression of NM2A (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH9 (Myh9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-WPRE-bGH-DEST (JDW 1309)

Plasmid

#229815

Purpose

A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-His-APEX2

Plasmid

#201471

Purpose

Recombinant soluble APEX2 for SLAPSHOT labeling

Depositor

Yuh-Nung Jan

Article

Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

Insert

APEX2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT2

Plasmid

#209974

Purpose

Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Terminator (T_pepN)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGM79

Plasmid

#220171

Purpose

CRISRPi guide RNA against MARCHF5

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

MARCHF5 (sgRNA) (MARCHF5 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk3a

Plasmid

#208985

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3a (kcnk3a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-SOS

Plasmid

#208278

Purpose

Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-EGFR

Plasmid

#208281

Purpose

Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-EGFR

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTCF79 H-2D(b) (CD8 mut)

Plasmid

#180453

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2D(b) (CD8 mut)

Tags

BSP41

Expression

Bacterial

Mutation

D227K, replacement of transmembrane domain with e…

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-PIP5K1A

Plasmid

#202743

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ4-(HA)3

Plasmid

#198180

Purpose

Expression of HA-tagged AP-4 μ4 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-4 μ4

Tags

triple HA tag

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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