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pESC-MET

Plasmid

#222482

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Publication

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LYS

Plasmid

#222483

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Publication

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-ADE (V5 and HA)

Plasmid

#222489

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Publication

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Expression

Yeast

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SYS1-msGFPx3

Plasmid

#177112

Purpose

Expresses Sys1-msGFP3 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SYS1 (SYS1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS313-Sad1-Wt

Plasmid

#90308

Purpose

yeast expression

Depositor

Christine Guthrie

Publication

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

no tag

Expression

Yeast

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Publication

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pWZ699

Plasmid

#208117

Purpose

Payload yeast assembly vector with TK-mActb on backbone

Depositor

Jef Boeke

Publication

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Type

Empty backbone

Use

Synthetic Biology; Copy number inducible

Expression

Bacterial, Mammalian, and Y…

Promoter

Human PGK1 for thymidine kinase

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LOV-Turbo-V5_pRS415

Plasmid

#199667

Purpose

expresses LOV-Turbo in the yeast cytosol

Depositor

Alice Ting

Publication

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Tags

V5

Expression

Yeast

Promoter

Gal1,10

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pC-BAR-YR

Plasmid

#61766

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

Depositor

Ken Haynes

Publication

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Bar gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-TAF15 c1222t No Stop

Plasmid

#84900

Purpose

DONOR vector for Gateway cloning of TAF15 c1222t No Stop

Depositor

Aaron Gitler

Publication

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway Cloning

Mutation

c1222t No Stop

Promoter

none

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pLS410

Plasmid

#239123

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Publication

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHES943

Plasmid

#112641

Purpose

yeast mating pheromone response pathway reporter

Depositor

Hana El-Samad

Publication

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

yeGFP

Expression

Yeast

Promoter

AGA1

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pACT2-Kre33

Plasmid

#99990

Purpose

Express yeast Kre33 with Y2H activation domain fusion

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Esf1

Plasmid

#99987

Purpose

Express yeast Esf1 with Y2H binding domain fusion

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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p414GPD_Hsp82_CycTER

Plasmid

#61710

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

GPD

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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p414ADH_Hsp82_noTER

Plasmid

#61715

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

ADH1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSW149

Plasmid

#122248

Purpose

pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

Depositor

Sarah Walker

Publication

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

Intein-Chitin-binding domain

Expression

Bacterial

Promoter

T7

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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