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Hy_pMT dati MCS circfirefly_700

Plasmid

#110115

Purpose

Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_500

Plasmid

#110114

Purpose

Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCas8

Plasmid

#82380

Purpose

dCas9 without a promoter in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

none

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1P-natMX6-pcr2P

Plasmid

#80620

Purpose

to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-P
PCR2 from mat1-P

Expression

Yeast

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1M-natMX6-pcr2M

Plasmid

#80621

Purpose

to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-M
PCR2 from mat1-M

Expression

Yeast

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-nMyc-m1_3'UTR

Plasmid

#31859

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc mutant-1 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

first Let-7c Seed changed from TACCTC to TAACGC

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
phtsResA

Plasmid

#25914

Depositor

Johann Deisenhofer

Article

Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

Insert

His-tagged soluble ResA (resA Bacillus subtilis)

Tags

His

Expression

Bacterial

Mutation

Start at Serine 37 to remove n-terminal transmemb…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
CJ38 (miR-1006 seed mutant perfect site)

Plasmid

#17771

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1006 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ39 (miR-1003 seed mutant perfect site)

Plasmid

#17772

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1003 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
HI0058/pET-21a(+)c-tag

Plasmid

#13097

Depositor

SeWon Suh

Article

Ku et al Acta Crystallogr D Biol Crystallogr. 200

Insert

3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Radical Fringe (CT#475)

Plasmid

#13981

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (B) Radical Fringe (CT#476)

Plasmid

#13982

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (B) flag Radical Fringe (CT#472)

Plasmid

#13983

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Radical Fringe (RFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
FUW-Luc-mCherry-puro

Plasmid

#183685

Purpose

Lentiviral vector expressing luciferase and mCherry.

Depositor

Andrew Kung

Article

Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

Insert

Luc-E2A-mCherry-T2A-PuroR

Use

Lentiviral

Promoter

Human Ubiquitin C (UbC) promoter

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC19-13XLexAop2-IVS-myr::GFP

Plasmid

#26224

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

myr::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-TNFalpha

Plasmid

#28089

Depositor

Jennifer Stow

Article

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

TNFalpha (Tnf Mouse)

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

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pAL750 :: msfGFP

Plasmid

#250661

Purpose

Inducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcanii

Depositor

Alexandre Bisson

Article

Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

Insert

Monomeric Superfolder Green Fluorescent Protein

Use

Haloarchaeal expression

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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3XMEF2-luc

Plasmid

#32967

Purpose

Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

Depositor

Ron Prywes

Insert

MEF2 binding sites (MEF2A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

MEF2

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-mCD8::GFP

Plasmid

#26220

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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