We narrowed to 32,755 results for: eng

1. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

   Plasmid

   #197028

   Purpose

   AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enOsCas12f1
   

   Use

   AAV

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227898

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227899

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227890

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227907

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60227

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227895

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RTN4 homology arms with mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227906

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227923

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227908

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227909

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227910

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLT

    Plasmid

    #227912

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    ATP1B3 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT

    Plasmid

    #227913

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pML454_G3BP1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227915

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    G3BP1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227916

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227891

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    VPS35 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227892

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227900

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge