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[pSK478] pLJC5 HA-Depdc5(P)

Plasmid

#109347

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant P

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

YDLLP(775-779)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(K271Q)

Plasmid

#133270

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Mutation

K271Q

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP_hBrg1 (J1 antibody)

Plasmid

#26109

Depositor

Jerry Crabtree

Article

Wang et al EMBO J. 1996 Oct 1. 15(19):5370-82.

Insert

Brg1 (SMARCA4 Human)

Tags

MBP

Expression

Bacterial

Available Since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R

Plasmid

#127135

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K230R

Plasmid

#127134

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227R

Plasmid

#127133

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK CHAC1 3'UTR

Plasmid

#19861

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

CHAC1 3'UTR (CHAC1 Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-TAN1 vpr

Plasmid

#115819

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (TAN1)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

Plasmid

#56658

Purpose

expresses constitutively active human PKCi with a deletion on the PB1 domain

Depositor

Pedro Salas

Article

Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

Insert

Human PKCi (PRKCI Human)

Use

Lentiviral

Tags

V5/His

Mutation

A120E and deletion of PB1 domain (aa 24–98)

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 R133A/P139A (#626)

Plasmid

#99946

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

R133A/P139A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(deltaP)

Plasmid

#83507

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

delta . Deletion of residues 187-283

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pmcsg7-mCRT(deltaC)

Plasmid

#83506

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

delta C. Contains residues 1-339

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA1 3XFlag ATXN1[15Q]-delAXH

Plasmid

#33241

Depositor

Huda Zoghbi

Article

Lam et al Cell. 2006 Dec 29;127(7):1335-47.

Insert

ATXN1[2Q]-delAXH (ATXN1 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
HOXC4 (human) MSCV

Plasmid

#8546

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXC4 (HOXC4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains improved ribosomal binding site and NcoI…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLENTI_RPS3_K214R_Keima

Plasmid

#127143

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K214R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b-hMED9

Plasmid

#15365

Depositor

Joan Conaway, Ronald Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pCI-Neo-gp78/2853

Plasmid

#11435

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

Plasmid

#127363

Purpose

Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPFAS-2XStrep

Plasmid

#125682

Purpose

expresses human PFAS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PFAS (PFAS Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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