We narrowed to 15,981 results for: LEA

1. pAAV-EF1a-Flpo

   Plasmid

   #55637

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV2

   Insert

   Flpo
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-CRISPRv2-sgNTC13-hygro

   Plasmid

   #231988

   Purpose

   Knockout non-targeting control

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 and hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nLuc_AP4_TEVs_P3mS

   Plasmid

   #119299

   Purpose

   Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
   

   Use

   Luciferase

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 4_T7-mScarlet3-RitC

   Plasmid

   #225931

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-RitC
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG_iPE-N

   Plasmid

   #214734

   Purpose

   encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-N_bGH
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-actin

   Plasmid

   #118378

   Purpose

   Gateway compatible donor vector with WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-TPH2-Cre

   Plasmid

   #189616

   Purpose

   Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Cre Recombinase
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Tph2

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG_iPE-C

   Plasmid

   #214735

   Purpose

   encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-C_bGH
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-mCherry-IRES-Dre

   Plasmid

   #55633

   Purpose

   Expresses Dre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Inserts

   mCherry
   Dre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-EF1a-GalT-IRES-Puromycin

    Plasmid

    #134862

    Purpose

    Expresses EGFP-tagged Golgi marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    beta 1,4-galactosyltransferase (B4GALT1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 83-398

    Promoter

    EF1a

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

    Plasmid

    #176280

    Purpose

    Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBac-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SpRY-ABE8e(V106W)

    Plasmid

    #198553

    Purpose

    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CRISPRv2-sgNTC49-puro

    Plasmid

    #231989

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Tags

    DHFR F[3]

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid

    #181743

    Purpose

    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-SUMO-Rad23A_pET11a

    Plasmid

    #169124

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hRad23A (RAD23A Human)
    

    Expression

    Bacterial

    Mutation

    wt

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUG-Pten

    Plasmid

    #140732

    Purpose

    Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-Pten (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    hUbiC

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STAT3(Y705F)-TAL-Luc

    Plasmid

    #46933

    Purpose

    Luciferase Reporter for mutant STAT3

    Depositor

    Afshin Dowlati

    Article

    Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

    Insert

    STAT3(Y705F) (STAT3 Human)
    

    Use

    Luciferase

    Tags

    FLAG

    Mutation

    Tyrosine 705 to Phenylalanine

    Promoter

    TA Luciferase

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only