Addgene

We narrowed to 15,358 results for: LEA

Showing: 6281 - 6300 of 15358 results

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2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-STChRger2-TS-EYFP

Plasmid

#129395

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pL90-Nat-2xHO

Plasmid

#139069

Purpose

CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Insert

Two guide RNAs targeting the HO endonuclease

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TDH3

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Use

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTER E2F2shRNA human

Plasmid

#66884

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F transcription factor 2 (E2F2 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Tet

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pVC297 VEGF Site#1

Plasmid

#47505

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 1 (VEGFA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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ADGRL3-CTF-Flag

Plasmid

#201219

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Plasmid

#210504

Purpose

expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

Plasmid

#127865

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

deleted the c-terminal 20 amino acids

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-B103 (LM2907)

Plasmid

#208968

Purpose

Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

B103-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVC228 VEGF Site#3

Plasmid

#47507

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 3 (VEGFA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_EE-NG-nCas9

Plasmid

#163542

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

EE-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Promoter

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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nLuc_AP4_TEVs_P3mS

Plasmid

#119299

Purpose

Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker

Use

Luciferase

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-APPL1-ΔPTB

Plasmid

#59768

Purpose

Expresses APPL1 with PTB domain deleted

Depositor

Donna Webb

Article

Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

Insert

APPL1 (APPL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 467-709

Promoter

CMV

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago2

Plasmid

#10821

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

R668H. Additionally, N terminus different from wi…

Promoter

Available since

Oct. 14, 2005

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75232

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-cytoBirA-2a-mCherry

Plasmid

#79885

Purpose

Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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