We narrowed to 7,679 results for: URE

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actc1b-mKate2-Dnm2b-DN

Plasmid

#109522

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dnm2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-MTM1

Plasmid

#109491

Purpose

Muscle specific zebrafish expression of mKate2 fused to myotubularin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

MTM1 (mtm1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3B-3'UTR

Plasmid

#136044

Purpose

UPF3B shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CAGGGCAAAGAATAGAGAGAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3B (UPF3B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-148-464

Plasmid

#136021

Purpose

EIF3B (148-464) 3'UTR fragment inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-465-552

Plasmid

#136022

Purpose

EIF3B (465-552) 3'UTR fragment inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-148-552

Plasmid

#136024

Purpose

EIF3B (148-552) 3'UTR fragment inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19_TTE1564_UUCG-loop

Plasmid

#133581

Purpose

T7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitch

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

P2-deletion mutant of Tte preQ1-riboswitch

Use

Unspecified

Mutation

replacement of riboswitch nucleotides U6 through …

Promoter

T7

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-EGFP

Plasmid

#132974

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

eGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD168

Plasmid

#121916

Purpose

Oth-NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

I111K, E116K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pABD933

Plasmid

#67917

Purpose

Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta

Depositor

Bruce Downie

Article

Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

Insert

CTG10

Use

Gateway Cloning

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only
pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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