We narrowed to 11,947 results for: emb

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pLPT2

Plasmid

#209974

Purpose

Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Terminator (T_pepN)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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FKBP-GFP-LAP2beta

Plasmid

#222416

Purpose

To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Publication

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LAP2beta (Tmpo Rat)

Tags

FKBP-GFP

Expression

Mammalian

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGM79

Plasmid

#220171

Purpose

CRISRPi guide RNA against MARCHF5

Depositor

Jonathan Weissman

Publication

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

MARCHF5 (sgRNA) (MARCHF5 Human)

Use

Lentiviral

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Publication

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA063

Plasmid

#216020

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nCas9-SpRY (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA062

Plasmid

#216019

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nCas9-SpG (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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P2RY8-DuET

Plasmid

#213365

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

P2RY8 (P2RY8 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk3a

Plasmid

#208985

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Publication

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3a (kcnk3a Zebrafish)

Use

Cloning

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-SOS

Plasmid

#208278

Purpose

Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-EGFR

Plasmid

#208281

Purpose

Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-EGFR

Use

Lentiviral

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTCF79 H-2D(b) (CD8 mut)

Plasmid

#180453

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2D(b) (CD8 mut)

Tags

BSP41

Expression

Bacterial

Mutation

D227K, replacement of transmembrane domain with e…

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa LCD

Plasmid

#197014

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Publication

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Publication

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_cyto

Plasmid

#182817

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Kaede-SpVasa

Plasmid

#185582

Purpose

for photoconversion

Depositor

Mamiko Yajima

Publication

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

Kaede

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-mCherry-6H

Plasmid

#187104

Purpose

Protein Expression

Depositor

Philipp Niethammer

Publication

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

mCherry

Expression

Bacterial

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-TagRFP-T-ps

Plasmid

#178656

Purpose

Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

Depositor

Jeffrey Gerst

Publication

Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

Insert

TagRFP-T with palmitoylation signal at N-terminus

Use

Lentiviral

Tags

Palmitoylation signal

Promoter

Ubc

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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