We narrowed to 7,027 results for: 11

1. pNAS1b split mCherry 14-3-3β

   Plasmid

   #111880

   Purpose

   Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Use

   Tags

   Split mCherry system: 14-3-3β fused to C-terminal…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Actin.CIB.GFP

   Plasmid

   #133618

   Purpose

   Expresses beta actin - CIBN - GFP protein fusion

   Depositor

   Robert Hughes

   Article

   Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

   Insert

   betaActin - CIB
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNAS1b split mCherry 14-3-3σ

   Plasmid

   #111881

   Purpose

   Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Use

   Tags

   Split mCherry system: 14-3-3σ fused to C-terminal…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ActinS14V.CIB.GFP

   Plasmid

   #133620

   Purpose

   Expresses beta actin S14V - CIBN - GFP protein fusion

   Depositor

   Robert Hughes

   Article

   Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

   Insert

   betaActin - CIB
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY71-tet.I.8

   Plasmid

   #133521

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV4-Flag-mIL-17RA/YFP.Hygro

   Plasmid

   #46862

   Purpose

   encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA-YFP
   

   Use

   Tags

   Flag/IL-3 signal sequence and fused to CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

7. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCellFree_G03 FOXM

   Plasmid

   #67118

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   FOXM (FOXM1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGEMHE:hTRAAK(Q258K)

   Plasmid

   #133077

   Purpose

   X. laevis expression vector. It will generate the human TRAAK channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Q258K

   Promoter

   T7

   Available since

   Oct. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNAS1b split mCherry NEDD4-2 WW2

    Plasmid

    #111883

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: NEDD4-2 WW2 fused to C-term…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b-ITPA

    Plasmid

    #60824

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Porta et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    ITPA (ITPA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. binder/tag-WT-FAK-insertionA

    Plasmid

    #190439

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    FAK (Ptk2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SsrA inserted after aa 35

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK-HA-TRAF4

    Plasmid

    #58314

    Purpose

    HA-tagged mouse TRAF4

    Depositor

    Ying Zhang

    Article

    Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

    Insert

    TNF receptor associated factor 4 (Traf4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET15b-At 14-3-3 phi

    Plasmid

    #44939

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 phi (GF14 PHI Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pRAN357

    Plasmid

    #120898

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYZ_NAND2_L17_S19_S11

    Plasmid

    #132730

    Purpose

    T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND2_L17_S19_S11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15b-At 14-3-3 lambda

    Plasmid

    #44942

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 lambda (GRF6 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCDEF3‐mTim1 (no tag)

    Plasmid

    #49206

    Purpose

    expresses mouse Tim1

    Depositor

    Lawrence Kane

    Article

    de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

    Insert

    Tim1 (Havcr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCMV4-Flag-mIL-17RA/CFP.Hygro

    Plasmid

    #46861

    Purpose

    encodes Flag-tagged mouse IL-17RA fused to Cyan Fl. Protein

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-CFP
    

    Use

    Tags

    Flag/IL-3 signal sequence and fused to CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only