We narrowed to 14,352 results for: Cas9
-
Plasmid#128123PurposeCo-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA HEK + GFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_tdtomato_reporter_1_ST1_2x_binding
Plasmid#79368PurposeAssays activity of transcriptional activators fused to ST1 Cas9DepositorInserttdtomato
UseCRISPRExpressionMammalianAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTX201
Plasmid#89265PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA02DepositorInsertOsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTX198
Plasmid#89262PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01DepositorInsertOsPDS-gRNA01
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTX168
Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGLOW30
Plasmid#172697PurposeC. elegans mScarlet co-injection markerDepositorInsertPmyo-2::mScarlet
TagsmScarletExpressionWormPromotermyo-2Available sinceSept. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV ORANGE Gria1-HaloTag KI
Plasmid#139655PurposeEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)DepositorInsertgRNA and HaloTag donor
UseAAVExpressionMammalianPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF279
Plasmid#167674PurposeIntermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS-GMR-eya(shRNA)
Plasmid#157991PurposepBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.DepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere
Plasmid#85715PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInserttelomere sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_GAL4UAS-Luciferase reporter
Plasmid#64157PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBC2101-reci
Plasmid#117170PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLSDepositorInsertreci_wtCas9 with SV40 NLS
UseLentiviralAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC2102-reci
Plasmid#117171PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLSDepositorInsertreci_wtCas9 without NLS
UseLentiviralPromoterU6 PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTaU6-esgRNA
Plasmid#115630Purposeexpression esgRNA in wheat protoplastsDepositorPublicationInsertTaU6p-esgRNA
UseCRISPRAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR and Gateway CloningPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJA50
Plasmid#59931PurposegRNA for cleavage at unc-58(e665) locus in C elegansDepositorInsertunc-58(e665) gRNA
UseCRISPRPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC49
Plasmid#104823PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma17g11235
UseCRISPRExpressionPlantAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HR donor
Plasmid#97317PurposeHR donor for fusing a p2A-mCherry reporter to mouse Actb.DepositorInsertActb HR donor
UseAAV and Mouse TargetingExpressionMammalianAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by