We narrowed to 30,307 results for: PLE

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pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoVL-lexA

Plasmid

#138406

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

V2 tail-TEVcs(ENLYFQL)-LexAp65

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pT7goldRNA

Plasmid

#47930

Purpose

In vitro transcription of golden gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

golden gRNA (slc24a5 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoVG-lexA

Plasmid

#138405

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

V2 tail-TEVcs(ENLYFQG)-LexAp65

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMZ222

Plasmid

#52223

Purpose

Constitutive expression of humanized Cas9

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

Cas9

Expression

Yeast

Mutation

silent mutation in the original CspCI site

Promoter

adh1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Vps20_deltaC

Plasmid

#21491

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

Vps20 Delta_C (VPS20 Budding Yeast)

Tags

6x His and MBP

Expression

Bacterial

Mutation

bp 511-663 deleted

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pUPD2-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211862

Purpose

Mutant E3-DART ligase in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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NTERY00

Plasmid

#184109

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY00

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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NTERY02

Plasmid

#184120

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY02

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoL-lexA

Plasmid

#138404

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

TEVcs(ENLYFQL)-LexAp65

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTYB2-PAB1

Plasmid

#37234

Depositor

Jon Lorsch

Article

Park et al EMBO J. 2011 Jan 19;30(2):302-16. Epub 2

Insert

PAB1 (PAB1 Budding Yeast)

Tags

intein

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pET11a-dH2B

Plasmid

#105501

Purpose

Expression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH2B

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pF3BGX-TangoG-lexA

Plasmid

#138403

Purpose

RMCE vector for generating C-terminal Tango fusion

Depositor

Shu Kondo

Article

Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

Insert

TEVcs(ENLYFQG)-LexAp65

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Tags

HA tag nuoG

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRS513

Plasmid

#17283

Depositor

Nancy Craig

Article

Skelding et al EMBO J. 2002 Jul 1. 21(13):3494-504.

Insert

TnsB (tnsB E. coli transposon Tn7)

Tags

Chitin-binding domain-intein

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

Plasmid

#32143

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

RSRT> STOP STOP RSRT>

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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NTERY01

Plasmid

#184119

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY01

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm) -R

Plasmid

#48888

Purpose

Negative feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pHM

Plasmid

#64173

Purpose

harbours marker genes HIS3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP211-Lenti- (MCS)-WPRE-pA

Plasmid

#63083

Purpose

plenti vector containing MCS-WPRE-pA. Contains no promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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