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BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTac-A control-luci

Plasmid

#65939

Purpose

No-Aptamer Control for Theophylline Riboswitch A with luciferase gene

Depositor

Justin Gallivan

Article

Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

Insert

luciferase

Expression

Bacterial

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pME_G_E_004_UAV_mScarlet-I

Plasmid

#235974

Purpose

Universal acceptor vector with mScarlet-I placeholder for lvl0 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

UAV_mScar

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pnEA-NpHG_N

Plasmid

#147033

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvS_M

Plasmid

#146926

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X2

Plasmid

#176152

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X3

Plasmid

#176153

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTac-A control-luci

Plasmid

#65939

Purpose

No-Aptamer Control for Theophylline Riboswitch A with luciferase gene

Depositor

Justin Gallivan

Article

Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

Insert

luciferase

Expression

Bacterial

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pTE5607

Plasmid

#250513

Purpose

pSecYEG; cell-free expression of functional SecYEG

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-secYEG

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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MCS_BsmBI

Plasmid

#241170

Purpose

Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

MCS; BsmBI

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3b_029_mScarlet-I

Plasmid

#235882

Purpose

mScarlet-I N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mScarlet-I N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pVEntry

Plasmid

#207674

Purpose

Entry vector for Trio vector system, with vanillate-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPEntry

Plasmid

#207673

Purpose

Entry vector for Trio vector system, with DAPG-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge