We narrowed to 1,173 results for: 1302

1. pJC45BS104 F130W

   Plasmid

   #1302

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 F130W (HSP104 Budding Yeast)
   

   Tags

   10X His tag

   Expression

   Bacterial

   Mutation

   Changed Phe 130 to Trp

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pVYD

   Plasmid

   #61302

   Purpose

   Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

   Type

   Empty backbone

   Tags

   SYFP2

   Expression

   Bacterial

   Promoter

   PV10

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFTK030

   Plasmid

   #171302

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   11xQUAS UAS (for fusion)
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p413GPD-hTPI Ile170Val

   Plasmid

   #50720

   Purpose

   expression of the human TPI Ile170Val allele in yeast

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Expression

   Yeast

   Mutation

   Isoleucine 170 to Valine

   Promoter

   GPD

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET20b- hTPI Ile170Val

   Plasmid

   #50724

   Purpose

   overexpression of the human TPI1 Ile170Val allele in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   changed Isoleucine 170 to Valine

   Promoter

   T7

   Available Since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-Kan_FR13_00067231

   Plasmid

   #121302

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   FR13_00067231
   

   Use

   Golden gate

   Mutation

   Magnaporthe oryzae

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p413GPD-hTPI Lys13Arg

   Plasmid

   #50722

   Purpose

   expression of the human TPI Lys13Arg allele in yeast

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Expression

   Yeast

   Mutation

   changed Lysine 13 to Argenine

   Promoter

   GPD

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p413GPD-hTPI Ile170Thr

   Plasmid

   #50721

   Purpose

   expression of the human TPI Ile170Thr allele in yeast

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Expression

   Yeast

   Mutation

   Isoleucine 170 to Threonine

   Promoter

   GPD

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TAL3128

   Plasmid

   #41302

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-nptx2a-Left (nptx2a Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pET20b- hTPI Ile170Thr

   Plasmid

   #50725

   Purpose

   overexpression of the human TPI1 Ile170Thr allele in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   changed Isoleucine 170 to Threonine

   Promoter

   T7

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid

    #185294

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

    Plasmid

    #185300

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2_PARRC_98-130
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

    Plasmid

    #185296

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA_1-208
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ApoE3_47-300_mCh-SspB (pBS1070)

    Plasmid

    #185293

    Purpose

    For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_47-300
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ApoE4_47-300_mCh-SspB (pBS1069)

    Plasmid

    #185292

    Purpose

    For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE4_47-300
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ApoE2_47-300_mCh-SspB (pBS1068)

    Plasmid

    #185291

    Purpose

    For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE2_47-300
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_STAC-2/2

    Plasmid

    #91302

    Purpose

    Protein expression and purification of human SH3 domain construct STAC-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAC-2/2 (STAC Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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