Skip to main content
Addgene
Showing: 621 - 640 of 874 results

  5. RT-4

    Plasmid
    #220443
    Purpose
    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.
    Depositor
    Marvin Wickens
    Article
    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do
    Insert
    PUF3 (PUF3 Budding Yeast)
    Use
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220443-plasmid-map-sequence-id-436941

    Enlarge

  10. YCp33-YDL183c-HA

    Plasmid
    #70164
    Purpose
    yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end
    Depositor
    Karin Nowikovsky
    Article
    Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41
    Insert
    YDL183c
    Use
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Promoter
    YDL183c (217 nucleotides upstream of the ATG)
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

    Enlarge

Showing: 621 - 640 of 874 results