We narrowed to 740 results for: SG

1. pAAV_COL4A5_self-cleavingCas9

   Plasmid

   #130282

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLAP-BUBR1-K795A

   Plasmid

   #114061

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

   Insert

   BUBR1-K795A (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-K795A

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIREN-RetroQ-HSPE-sh1

   Plasmid

   #92033

   Purpose

   pSIREN-RetroQ vector containing shRNA sequence to HPSE

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA #1 to HPSE (Hpse Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSIREN-RetroQ-HSPE-sh2

   Plasmid

   #92034

   Purpose

   pSIREN-RetroQ vector containing shRNA sequence to HPSE

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA #2 to HPSE (Hpse Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-AUP1-FLAG-DATDCUE

   Plasmid

   #185340

   Purpose

   Assessing domain requirement sof AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 90-337 corresponding to the acyl t…

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3Bi-ctd-Cas9n-UGI-NLS

   Plasmid

   #109426

   Purpose

   Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-NDUFA5-mVenus

   Plasmid

   #185646

   Purpose

   NDUFA5 Complex I sub-unit with C-terminus mVenus tag

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   NDUFA5 (NDUFA5 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM30-ARC105

    Plasmid

    #133426

    Purpose

    Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 5-78 fused to C-terminal of …

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

    Plasmid

    #69114

    Purpose

    Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L1939A and L1942A amino acid substitutions. Sile…

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-NDUFV2-mVenus

    Plasmid

    #185653

    Purpose

    NDUFV2 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid

    #109430

    Purpose

    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-NDUFS6-mVenus

    Plasmid

    #185806

    Purpose

    NDUFS6 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFS6 (NDUFS6 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-NDUFA7-mVenus

    Plasmid

    #185647

    Purpose

    NDUFA7 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFA7 (NDUFA7 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVTHM-Bptf-sh28

    Plasmid

    #83278

    Purpose

    shRNA targeting BPTF

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #28 to BPTF (BPTF, Bptf Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE-H1

    Available since

    Oct. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLVTHM-Bptf-sh5

    Plasmid

    #83277

    Purpose

    shRNA targeting BPTF

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #5 to BPTF (BPTF, Bptf Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE-H1

    Available since

    Oct. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGEX-2TK-arc105

    Plasmid

    #133425

    Purpose

    Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-95 or ARC105 fused to the …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6P-1-HRD1 aa 236-444

    Plasmid

    #185349

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    HRD1/SYVN (SYVN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 236-444 only

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-6P-1 AUP1 292-410

    Plasmid

    #185331

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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