We narrowed to 20,107 results for: NSI

Showing: 6401 - 6420 of 20107 results

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pET28a_6HT-TBP6.7(R49A)

Plasmid

#112726

Purpose

Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R49A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R49A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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TRS6E1b-luc(-732/-721) mutant 5

Plasmid

#45388

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -725/723

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-725/-723 mutated from gtt to ccc

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm1

Plasmid

#20878

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 mutation (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_FUT8-1/1

Plasmid

#91270

Purpose

Protein expression and purification of human SH3 domain construct FUT8-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FUT8-1/1 (FUT8 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP4

Plasmid

#129502

Purpose

Expresses AausFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP4

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMADM-alpha

Plasmid

#36890

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, deleted…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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p270 pCMV-CRE-M-AR(LNCaP)

Plasmid

#12496

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

Thr 868 Ala in AR-LBD

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
MAC_N_CDK7

Plasmid

#111684

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CDK7 (CDK7 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD

Plasmid

#140304

Purpose

Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-KD

Plasmid

#140296

Purpose

Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD-KD

Plasmid

#140300

Purpose

Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

lacks the SH3 and SH2 domains

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp

Plasmid

#108481

Purpose

inducible expression of vsv-CenpB-1-166-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(2Q)

Plasmid

#21752

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm2

Plasmid

#20879

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Predicted miRNA binding site 2 (Position ~1100) G…

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only
pQC hKGA.wt V5-His IRES G418

Plasmid

#110390

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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DPYS-FLAG

Plasmid

#61618

Purpose

To study the effect of DPYS activity on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidinase (DPYS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK2-1/1

Plasmid

#91482

Purpose

Protein expression and purification of human SH3 domain construct DOCK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK2-1/1 (DOCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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