We narrowed to 20,399 results for: OMP;

1. PX429 SpCas9 N863A nickase

   Plasmid

   #68706

   Purpose

   SpCas9 N863A nickase

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   SpCas9 N863A nicking mutant
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nuc-ABKAR

   Plasmid

   #61511

   Purpose

   Expression of nuclear AMPK biosensor

   Depositor

   Takanari Inoue, Jin Zhang

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Tags

   NLS (see comments)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pGEMT-PT2A-iRFP670-Tdtomato-GFP

   Plasmid

   #111817

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   Tdtomato
   GFP
   

   Use

   Cloning intermediate

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

   Plasmid

   #199356

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPlit-OC-hDib1

   Plasmid

   #58789

   Purpose

   Express hDib1-LucC fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   hDib1 (TXNL4A Human)
   

   Tags

   Flag and Luciferase (394-550aa)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pGUS (GB0208)

   Plasmid

   #68203

   Purpose

   Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   GUS coding region
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin beta1(1-455)-tev-BHCys

   Plasmid

   #27315

   Depositor

   Timothy Springer

   Article

   Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

   Insert

   integrin beta1 (ITGB1 Human)
   

   Expression

   Mammalian

   Mutation

   beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pISH-Barx1

   Plasmid

   #84306

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   Barx1 (Barx1 Mouse)
   

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW-AlphaLobe

   Plasmid

   #113255

   Purpose

   E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

   Depositor

   Jennifer Doudna

   Article

   Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   S. pyogenes Cas9 alpha-helical lobe
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   residues 56–714; See paper for description

   Promoter

   T7

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-PhoCl-mCherry-myc

    Plasmid

    #87692

    Purpose

    Express PhoCl-mCherry with C-term myc tag in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl-mCherry-myc
    

    Tags

    myc tag

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Golgi-ABKAR

    Plasmid

    #61507

    Purpose

    Expression of AMPK biosensor targeted to the Golgi

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Tags

    Golgi-targeting signal (see comments)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Marathon-RT tetramutant for Split-PE (pEH101)

    Plasmid

    #190109

    Purpose

    Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, D74R, N116K, N197R

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. YSBE 508 : pNEO_GFP

    Plasmid

    #92081

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    GFP
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b::mAID

    Plasmid

    #15923

    Depositor

    Nina Papavasiliou

    Article

    Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

    Insert

    AID (Aicda Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. EB3-mTurquoise2

    Plasmid

    #98825

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. JAF2.13

    Plasmid

    #45608

    Depositor

    Gregory Pazour

    Article

    Follit et al Mol Biol Cell. 2006 Sep . 17(9):3781-92.

    Insert

    IFT20 (Ift20 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. p3E-mTagBFP-CAAX

    Plasmid

    #75176

    Purpose

    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUPER IKKe

    Plasmid

    #26211

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    pSUPER IKK epsilon (IKBKE Human)
    

    Use

    RNAi

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

19. px458_2A_GFP_sgRNA_TRIM25_G1

    Plasmid

    #127119

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA TRIM25 (TRIM25 Human)
    

    Use

    CRISPR

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HBT_pcoCASphi_version2

    Plasmid

    #197932

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    2xNLS-2xFLAG

    Expression

    Plant

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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