We narrowed to 9,182 results for: mel

1. pEN35-CDKN2A-Ex2-R

   Plasmid

   #110737

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-ZNF736

   Plasmid

   #101602

   Purpose

   Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF736 (ZNF736 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKII-Chrimson-GFP

   Plasmid

   #62718

   Purpose

   AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chrimson-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159676

   Purpose

   Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mouse Oct4-GFP GOF18 transgenic reporter

   Plasmid

   #60527

   Purpose

   BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   GFPpA
   

   Expression

   Mammalian

   Promoter

   Oct4

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN35-CDKN2A-Ex2-L

   Plasmid

   #110736

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSELECT-HA-mFOXO1

   Plasmid

   #83308

   Purpose

   Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection marker

   Depositor

   Steven Abcouwer

   Article

   Kong et al Exp Eye Res. 2016 Aug 7;151:82-95. doi:

   Insert

   FoxO1 (Foxo1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hEF1/HTLV prom

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Circular 200,100 (mIDUA)

   Plasmid

   #170123

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Idua Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Circular 200,100 (GAPDH)

   Plasmid

   #170120

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (GAPDH Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRecLV105 MEF2C

    Plasmid

    #107240

    Purpose

    A lentiviral vector for expression in mammalian cells encoding MEF2C insert

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1α1.1-tdTomato

    Plasmid

    #122101

    Purpose

    AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Promoter

    EF1a（1.1kb short version)

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75114

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. anti-Notch1_E6-pBIOCAM5

    Plasmid

    #39344

    Depositor

    John McCafferty

    Article

    Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

    Insert

    scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Human, Mouse)
    

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX317-MBNL2

    Plasmid

    #115444

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    MBNL2 (MBNL2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-ZNF574

    Plasmid

    #88644

    Purpose

    Donor Vector containing ZNF574 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF574 (ZNF574 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-FLEX-fwd[Chrimson-tdT]

    Plasmid

    #59137

    Purpose

    Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson-tdTomato
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRecLV105 MEF2C S222A

    Plasmid

    #107239

    Purpose

    A lentiviral vector for expression in mammalian cells encoding MEF2C-S222A insert

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C S222A
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-ZNF251

    Plasmid

    #101451

    Purpose

    Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF251 (ZNF251 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75122

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    3x FLAG-tag

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

20. MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

    Plasmid

    #75115

    Purpose

    Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    deleted bromodomain

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only