We narrowed to 42,187 results for: ids

1. pAAV-nEF-SpCas9D10A nickase

   Plasmid

   #216736

   Purpose

   Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF-1-alpha core

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px552-U6-gRNA1-CMV-eGFP

   Plasmid

   #211758

   Purpose

   sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

   Depositor

   Glenn Yiu

   Article

   Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   gRNA1 targeting VEGF-A (VEGFA Mouse, Human, M. mulatta (rhesus macaque))
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. px552-U6-gRNA2-CMV-eGFP

   Plasmid

   #211759

   Purpose

   sgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

   Depositor

   Glenn Yiu

   Article

   Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   gRNA2 targeting VEGF-A (VEGFA Mouse, Human, M. mulatta (rhesus macaque))
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-CuO-V5-CASC3 siRes 110-480 188/218-mut

   Plasmid

   #158544

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Amino acids 110-480, C-terminal deletion, F188D, …

   Promoter

   CMV

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HL 5226

   Bacterial Strain

   #63667

   Purpose

   Lim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZ

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Bacterial Resistance

   None

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. DH10B-M.Osp807II

   Bacterial Strain

   #114267

   Purpose

   The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

   Depositor

   Christopher A. O'Callaghan

   Article

   Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

   Bacterial Resistance

   Bleocin (zeocin)

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

7. DH10B-M2.NmeMC58II

   Bacterial Strain

   #114268

   Purpose

   The strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus.

   Depositor

   Christopher A. O'Callaghan

   Article

   Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

   Bacterial Resistance

   Bleocin (zeocin)

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

8. DH10B-M.XmnI

   Bacterial Strain

   #114269

   Purpose

   The strain expresses M.XmnI methylase and zeocin selection marker from the arsB locus.

   Depositor

   Christopher A. O'Callaghan

   Article

   Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

   Bacterial Resistance

   Bleocin (zeocin)

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

9. HL5410

   Bacterial Strain

   #62820

   Purpose

   Lim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZ + Dhfq

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Bacterial Resistance

   None

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

10. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid

    #178320

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Inserts

    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid

    #71684

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOmnibac_ZZ_linker_ybbR_Tau MTBD

    Plasmid

    #159719

    Purpose

    Expresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Tau microtubule-binding domain only (amino acids 242-367) (MAPT Human)
    

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Mutation

    Only amino acids 242-367

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PGL4.11-COL1A1mSMAD

    Plasmid

    #230917

    Purpose

    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant potential SMAD bindin…

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214278

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-Q61L

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGL4.11-COL1A1mTEAD

    Plasmid

    #230916

    Purpose

    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase- luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    mutant potential TEAD bind elements AGGAAT to CTG…

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid

    #214279

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-T17N

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214280

    Purpose

    Express split-Rac1 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Rac1-Q61L

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge